Mogi T, Yamamoto H, Nakao T, Yamato I, Anraku Y
Mol Gen Genet. 1986 Jan;202(1):35-41. doi: 10.1007/BF00330513.
Two new mutants of E. coli K12, strains PT9 and PT32 were isolated, that were defective in proline transport. They had no high affinity proline transport activity, but their cytoplasmic membranes retained proline binding activity with altered sensitivity to inhibition by p-chloromercuribenzoate (pCMB). The lesion was mapped at the putP gene, which is located at min 23 on the revised E. coli genetic map (Bachmann 1983) as a composite gene in the proline utilization gene cluster, putP, putC, and putA, arranged in this order. The putC gene was shown to regulate the synthesis of proline dehydrogenase (putA gene product). Hybrid plasmids carrying the put region (Motojima et al. 1979; Wood et al. 1979) were used to construct the physical map of the put region. The possible location of the putP gene in the DNA segment was determined by subcloning the putP gene, genetic complementation, and recombination analyses using several proline transport mutants.
分离出了大肠杆菌K12的两个新突变体，即PT9和PT32菌株，它们在脯氨酸转运方面存在缺陷。它们没有高亲和力的脯氨酸转运活性，但其细胞质膜保留了脯氨酸结合活性，且对对氯汞苯甲酸（pCMB）抑制的敏感性发生了改变。该损伤定位在putP基因上，在修订后的大肠杆菌遗传图谱（Bachmann，1983）上，位于第23分钟处，是脯氨酸利用基因簇putP、putC和putA中的一个复合基因，按此顺序排列。已证明putC基因调节脯氨酸脱氢酶（putA基因产物）的合成。携带put区域的杂交质粒（Motojima等人，1979年；Wood等人，1979年）被用于构建put区域的物理图谱。通过对putP基因进行亚克隆、遗传互补以及使用几种脯氨酸转运突变体进行重组分析，确定了putP基因在DNA片段中的可能位置。
