含有肌苷、7-脱氮肌苷、杀结核菌素、脉孢菌素和7-脱氮脉孢菌素作为限制性内切酶HindII、SalI和TaqI底物的寡核苷酸双链体。
Oligonucleotide duplexes containing inosine, 7-deazainosine, tubercidin, nebularine and 7-deazanebularine as substrates for restriction endonucleases HindII, SalI and TaqI.
作者信息
Jiricny J, Wood S G, Martin D, Ubasawa A
出版信息
Nucleic Acids Res. 1986 Aug 26;14(16):6579-90. doi: 10.1093/nar/14.16.6579.
Abstract
Synthetic hexadecanucleotide duplexes containing a single purine nucleotide analogue in the recognition sites of the restriction endonucleases HindII, SalI and TaqI were used to investigate the restriction site determinants required by these enzymes for sequence recognition and phosphodiester bond cleavage. The enzymes were, in general, unaffected by changes introduced into the minor groove of the helix. SalI was found to be inhibited by the major groove modifications introduced into the fourth position of its recognition sequence GTCGAC. HindII and TaqI were, by contrast, able to cleave the sites containing the analogues at this position. TaqI and, to a lesser extent, HindII could also be shown to tolerate "mismatch analogues" at this site.
摘要
在限制性内切酶HindII、SalI和TaqI的识别位点中含有单个嘌呤核苷酸类似物的合成十六核苷酸双链体，被用于研究这些酶进行序列识别和磷酸二酯键切割所需的限制位点决定因素。一般来说，这些酶不受引入螺旋小沟的变化的影响。发现SalI会被引入其识别序列GTCGAC第四个位置的大沟修饰所抑制。相比之下，HindII和TaqI能够切割在该位置含有类似物的位点。TaqI以及在较小程度上的HindII，也能被证明在该位点容忍“错配类似物”。
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