Weinrich S L, Hruby D E
Nucleic Acids Res. 1986 Apr 11;14(7):3003-16. doi: 10.1093/nar/14.7.3003.
The nucleotide sequence of a 5.1 kilobase-pair fragment from the central portion of the vaccinia virus genome has been determined. Within this region, five complete and two incomplete open reading frames (orfs) are tightly-clustered, tandemly-oriented, and read in the leftward direction. Late mRNA start sites for the five complete orfs and one incomplete orf were determined by S1 nuclease mapping. The two leftmost complete orfs correlated with late polypeptides of 65,000 and 32,000 molecular weight previously mapped to this region. When compared with each other and with sequences present in protein data banks, the five complete orfs showed no significant homology matches amongst themselves or any previously reported sequence. The six putative promoters were aligned with three previously sequenced late gene promoters. While all of the nine are A-T rich, the only apparent consensus sequence is TAA immediately preceeding the initiator ATG. Identification of this tandemly-oriented late gene cluster suggests local organization of the viral genome.
已确定来自痘苗病毒基因组中部的一个5.1千碱基对片段的核苷酸序列。在该区域内，五个完整的和两个不完整的开放阅读框（ORF）紧密聚集、串联排列，并向左转录。通过S1核酸酶图谱确定了五个完整ORF和一个不完整ORF的晚期mRNA起始位点。最左边的两个完整ORF与先前定位到该区域的分子量为65,000和32,000的晚期多肽相关。当五个完整ORF相互比较以及与蛋白质数据库中的序列比较时，它们之间未显示出明显的同源匹配，也未与任何先前报道的序列匹配。六个推定的启动子与三个先前测序的晚期基因启动子进行了比对。虽然所有九个启动子都富含A-T，但唯一明显的共有序列是紧接起始密码子ATG之前的TAA。这个串联排列的晚期基因簇的鉴定表明了病毒基因组的局部组织方式。
