Hashemi Zahra, Mortazavi-Derazkola Sobhan, Biparva Pouria, Goli Hamid Reza, Sadeghian Fereshteh, Kardan Mostafa, Rafiei Alireza, Ebrahimzadeh Mohammad Ali
Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, School of Pharmacy , Mazandaran University of Medical Sciences, Sari, Iran .
Medical Toxicology and Drug Abuse Research Center (MTDRC), Birjand University of Medical Sciences, Birjand, Iran.
Iran J Pharm Res. 2020 Fall;19(4):306-320. doi: 10.22037/ijpr.2020.112523.13805.
Biogenic synthesis of silver nanoparticles (SNPs) has great attention of scientists, as it provides clean, biocompatible, non-toxic and inexpensive fabrication. In this study, leaf extract was used for synthesizing SNPs which reduces silver nitrate into silver zero-valent. SNPs were characterized by UV, FTIR, XRD, SEM-EDS, and TEM analysis. They were also examined for their biological activities. The presence of biosynthesized SNPs was characterized by UV-visible spectroscopy and also crystal nature of SNPs was identified with XRD analysis. FT-IR spectrum was used to confirm the presence of different functional groups in the biomolecules which act as a capping agent for the nanoparticles. The morphology of SNPs was explored using SEM and the presence of silver was confirmed by elemental analysis. The size of the nanoparticles was in the range of 20-50 nm determined by TEM. The green synthesized SNPs showed good antibacterial activities against both gram-negative and gram-positive bacteria and also in resistant clinically isolated pathogens. Furthermore, the green synthesized SNPs showed reliable anticancer activity on the gastric adenocarcinoma (AGS) and breast (MCF-7) cancer cell lines with little effect on normal (HFF) cells. The antioxidant activity of SNPs showed a significant effect on the scavenging of free radicals and iron chelating activity.
银纳米粒子（SNPs）的生物合成备受科学家关注，因为它提供了一种清洁、生物相容、无毒且廉价的制备方法。在本研究中，叶提取物被用于合成将硝酸银还原为零价银的SNPs。通过紫外可见光谱（UV）、傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、扫描电子显微镜-能谱分析（SEM-EDS）和透射电子显微镜（TEM）分析对SNPs进行了表征。还对它们的生物活性进行了检测。通过紫外可见光谱对生物合成的SNPs的存在进行了表征，并用XRD分析确定了SNPs的晶体性质。FT-IR光谱用于确认生物分子中作为纳米粒子封端剂的不同官能团的存在。使用扫描电子显微镜探索了SNPs的形态，并通过元素分析确认了银的存在。通过透射电子显微镜测定，纳米粒子的尺寸在20-50nm范围内。绿色合成的SNPs对革兰氏阴性菌和革兰氏阳性菌以及耐药的临床分离病原体均表现出良好的抗菌活性。此外，绿色合成的SNPs对胃腺癌（AGS）和乳腺癌（MCF-7）细胞系表现出可靠的抗癌活性，而对正常（HFF）细胞影响较小。SNPs的抗氧化活性对自由基清除和铁螯合活性有显著影响。
