Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology University of Campania "Luigi Vanvitelli", 80138 Naples, Italy.
Division of General Surgery, Department of Cardiothoracic and Respiratory Science, University of Campania "Luigi Vanvitelli", 80138 Naples, Italy.
Int J Environ Res Public Health. 2018 Aug 9;15(8):1695. doi: 10.3390/ijerph15081695.
For the management of Staphylococci coagulase-negative infection, often related to biofilm formation, rapid and accurate identification is necessary in choosing a correct antibiotic therapy. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is becoming increasingly important for bacterial identification over traditional methods. Our aim was to validate the use of MALDI to discriminate biofilm-producing strains. Clinical strains coming from suture wires were identified and their protein profiles were compared to that obtained from two ATCC reference strains (biofilm producer and non-producer). MALDI identified the eighteen isolates as , combining sixteen profiles with the biofilm producer and two with the non-producer, confirming the results of crystal violet assay. Our data highlight that MALDI can be considered a good tool to discriminate between biofilm-producer and non-producer strains of , thus helping to establish an effective antibiotic therapy.
对于凝固酶阴性葡萄球菌感染的治疗，由于其常与生物膜的形成有关，因此在选择正确的抗生素治疗方案时，快速、准确的鉴定是非常必要的。基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱（MS）在细菌鉴定方面正逐渐变得比传统方法更为重要。我们的目的是验证 MALDI 用于区分产生物膜菌株的应用。我们对来自缝线的临床菌株进行了鉴定，并将其蛋白图谱与两个 ATCC 参考菌株（产生物膜和不产生物膜）进行了比较。MALDI 将这 18 个分离株鉴定为 ，其中 16 个图谱与产生物膜株一致，2 个与不产生物膜株一致，这与结晶紫试验的结果一致。我们的数据表明，MALDI 可作为区分 产生物膜和不产生物膜株的良好工具，从而有助于制定有效的抗生素治疗方案。
