College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang, Nanjing, 210095, Jiangsu, People's Republic of China.
Eur J Nutr. 2018 Dec;57(8):2735-2745. doi: 10.1007/s00394-017-1539-3. Epub 2017 Sep 21.
The present study investigated whether dietary methionine supplementation might protect against intrauterine growth retardation (IUGR)-induced damage in the intestine of piglets.
Thirty normal birth weight (NBW) female piglets and sixty same-sex IUGR piglets were weaned at 21 days of postnatal age and fed the control diet (4.0 g methionine per kg of feed, NBW-CON, and IUGR-CON groups) or the methionine-supplemented diet (5.2 g methionine per kg of feed, IUGR-MET group) for 28 days (n = 6).
Piglets in the IUGR-CON group showed decreased average daily feed intake and average daily gain and an increased feed conversion ratio than those in the NBW-CON group. Compared with NBW-CON piglets, IUGR-CON piglets had decreased villus height (VH) and villus height-to-crypt depth ratio in both the jejunum and ileum. In addition, in comparison with the NBW-CON piglets, IUGR increased the concentration of malondialdehyde (MDA) and the index of apoptosis, while it decreased the concentrations of methionine and reduced glutathione (GSH), the ratio of reduced glutathione/oxidized glutathione (GSH/GSSG), and the protein expression of occludin (OCLN) in both the jejunum and ileum. Dietary methionine supplementation decreased the MDA and protein carbonyl concentrations and the apoptotic index, while it increased the VH level, methionine and GSH concentrations, GSH/GSSG ratio, and the OCLN protein expression in the jejunum of IUGR-MET piglets.
Methionine may have beneficial effects in improving intestinal integrity and oxidative status in IUGR weanling piglets.
本研究旨在探讨饲粮蛋氨酸补充是否可以预防宫内生长迟缓（IUGR）引起的仔猪肠损伤。
30 头正常出生体重（NBW）雌性仔猪和 60 头同性别 IUGR 仔猪在产后 21 天断奶，分别饲喂对照饲粮（NBW-CON 和 IUGR-CON 组，每千克饲粮含 4.0 g 蛋氨酸）或蛋氨酸补充饲粮（IUGR-MET 组，每千克饲粮含 5.2 g 蛋氨酸），28 天。
与 NBW-CON 组相比，IUGR-CON 组仔猪的平均日采食量和平均日增重降低，料重比升高。与 NBW-CON 仔猪相比，IUGR-CON 仔猪空肠和回肠的绒毛高度（VH）和绒毛高度与隐窝深度比均降低。此外，与 NBW-CON 仔猪相比，IUGR 增加了 MDA 和凋亡指数，降低了蛋氨酸和还原型谷胱甘肽（GSH）浓度，降低了 GSH/GSSG 比值，降低了空肠和回肠的 occludin（OCLN）蛋白表达。饲粮蛋氨酸补充降低了 MDA 和蛋白羰基浓度及凋亡指数，增加了 VH 水平、蛋氨酸和 GSH 浓度、GSH/GSSG 比值，以及 IUGR-MET 仔猪空肠的 OCLN 蛋白表达。
蛋氨酸可能对改善 IUGR 断奶仔猪的肠道完整性和氧化状态有有益作用。
