School of Pharmacy, The University of Texas at El Paso, 500 West University Avenue, El Paso, TX 79968, USA.
College of Health Sciences, University of Texas at El Paso, 500 West University Avenue, El Paso, TX 79968, USA.
IET Nanobiotechnol. 2018 Sep;12(6):836-840. doi: 10.1049/iet-nbt.2017.0284.
Silver (Ag) complexes of drugs and their nanosystems have great potential as antibacterials. Recently, an Ag complex of furosemide (Ag-FSE) has shown to be a promising antimicrobial. However, poor solubility of Ag-FSE could hamper its introduction into clinics. Therefore, the authors developed a nanosuspension of Ag-FSE (Ag-FSE_NS) for its solubility and antibacterial activity enhancement. The aim of this study was to introduce a novel nanoantibiotic with enhanced antibacterial efficacy. Ag-FSE_NS was prepared by precipitation-ultrasonication technique. Size, polydispersity index (PI) and zeta potential (ZP) of prepared Ag-FSE_NS were measured by dynamic light scattering, whereas surface morphology was determined using scanning electron microscopy (SEM). antibacterial activity was evaluated against , and using broth microdilution method. Size, PI and ZP of optimised Ag-FSE_NS1 were 191.2 ± 19.34 nm, 0.465 ± 0.059 and -55.7 ± 8.18 mV, respectively. SEM revealed that Ag-FSE_NS1 particles were rod or needle-like with smooth surfaces. Saturation solubility of Ag-FSE in NS increased eight-fold than pure Ag-FSE. Ag-FSE_NS1 exhibited two-fold and eight-fold enhancements in activity against and , respectively. The results obtained showed that developed Ag-FSE_NS1 holds a promise as a topical antibacterial.
银（Ag）配合物药物及其纳米系统具有很大的作为抗菌剂的潜力。最近，呋塞米（Ag-FSE）的 Ag 配合物已显示出作为有前途的抗菌剂的潜力。然而，Ag-FSE 的溶解度差可能会阻碍其引入临床。因此，作者开发了一种 Ag-FSE 的纳米混悬剂（Ag-FSE_NS）来提高其溶解度和抗菌活性。本研究的目的是引入一种具有增强抗菌功效的新型纳米抗生素。Ag-FSE_NS 通过沉淀-超声技术制备。通过动态光散射测量了制备的 Ag-FSE_NS 的粒径、多分散指数（PI）和 Zeta 电位（ZP），而表面形态则通过扫描电子显微镜（SEM）确定。使用肉汤微量稀释法评估了对 、 和 的抗菌活性。优化后的 Ag-FSE_NS1 的粒径、PI 和 ZP 分别为 191.2 ± 19.34nm、0.465 ± 0.059 和 -55.7 ± 8.18mV。SEM 显示 Ag-FSE_NS1 颗粒呈棒状或针状，表面光滑。NS 中 Ag-FSE 的饱和溶解度比纯 Ag-FSE 增加了 8 倍。Ag-FSE_NS1 对 和 的活性分别提高了 2 倍和 8 倍。结果表明，所开发的 Ag-FSE_NS1 有望成为一种局部抗菌剂。
