Kim Jee Young, Dao Thien T P, Song Kwangho, Park Sait Byul, Jang Hyeri, Park Min Kyoung, Gan Soo Ueng, Kim Yeong Shik
College of Pharmacy and Natural Product Research Institute, Seoul National University, Seoul 08826, Republic of Korea.
Civil Appellate Division, Seoul Central District Court, Seoul 06594, Republic of Korea.
Evid Based Complement Alternat Med. 2018 Jul 17;2018:7972916. doi: 10.1155/2018/7972916. eCollection 2018.
L., known as graviola, is an evergreen plant of the tropical regions and is a rich source of natural products. Graviola has various biological activities, and it is best known for its anticancer activity. This study aimed to investigate the effects of crude graviola extract on breast cancer cells; in particular, we aimed to identify an agent against triple negative breast cancer (TNBC). We used the TNBC MDA-MB-231 cell line as the experimental model and the ER(+) non-TNBC MCF-7 breast cancer cell line as the control. We identified annonaceous acetogenins, including annonacin isomers, characteristic to this plant by using liquid chromatography tandem mass spectrometry (LC/MS/MS). We observed a significant decrease in the cell viability in both cell lines within 48 h, whereas impaired cell motility and invasiveness were observed only in the MDA-MB-231 cell line. While the MCF-7 cells showed an ER-dependent mechanism of apoptosis, the apoptosis of MDA-MB-231 cells was governed by an intrinsic apoptotic pathway triggered by graviola leaf extract (GLE).
刺果番荔枝，又称巴西番荔枝，是一种热带地区的常绿植物，富含天然产物。刺果番荔枝具有多种生物活性，其中抗癌活性最为人所知。本研究旨在探究刺果番荔枝粗提物对乳腺癌细胞的影响；具体而言，我们旨在鉴定一种针对三阴性乳腺癌（TNBC）的药物。我们使用TNBC的MDA-MB-231细胞系作为实验模型，ER(+)非TNBC的MCF-7乳腺癌细胞系作为对照。我们通过液相色谱串联质谱（LC/MS/MS）鉴定了该植物特有的番荔枝内酯，包括番荔枝宁异构体。我们观察到两种细胞系在48小时内细胞活力均显著下降，而仅在MDA-MB-231细胞系中观察到细胞运动性和侵袭性受损。虽然MCF-7细胞显示出雌激素受体（ER）依赖性凋亡机制，但MDA-MB-231细胞的凋亡是由刺果番荔枝叶提取物（GLE）触发的内源性凋亡途径所调控。
