Wu Minfeng, Li Yan, Guo Dongjie, Kui Gang, Li Bin, Deng Yu, Li Fulun
Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.
Department of Traditional Chinese Medicine, The Ninth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200011, China.
Evid Based Complement Alternat Med. 2018 Jul 9;2018:9463295. doi: 10.1155/2018/9463295. eCollection 2018.
Chronic ulcer, including diabetic ulcer, varicose ulcer, and pressure ulcer, negatively affects patients' quality of life. As microbiology plays an important role in the mechanism of pathology for chronic wound healing, this study concentrates on microecology environment of the wound and how Traditional Chinese Medicine (TCM) regulates wound bacteria. . The study took wound samples from 35 patients and analyzed bacteria variation before and after TCM treatment by 16s rRNA sequencing. All samples were evaluated from aspects of -diversity, -diversity, and Simpson's Diversity index. . After total DNA extraction, PCR, and 16S rRNA sequencing of wound bacteria from 35 individuals, it was discovered that younger patients with shorter course of disease have a higher microbial diversity and were easier to recover from ulcers. Additionally, gender also played a vital role in wound healing, and a significant microbial diversity existed between male and female patients. . Patients with chronic ulcers achieved a positive effect after TCM treatment (skin-producing ointment). Mechanistically, TCM helped promote wound healing by regulating the wound microbiota.
慢性溃疡，包括糖尿病溃疡、静脉曲张性溃疡和压疮，会对患者的生活质量产生负面影响。由于微生物学在慢性伤口愈合的病理机制中起着重要作用，本研究聚焦于伤口的微生态环境以及中药如何调节伤口细菌。该研究采集了35例患者的伤口样本，并通过16s rRNA测序分析了中药治疗前后的细菌变化。所有样本均从α多样性、β多样性和辛普森多样性指数方面进行评估。在对35名个体的伤口细菌进行总DNA提取、PCR和16S rRNA测序后，发现病程较短的年轻患者微生物多样性较高，溃疡愈合更容易。此外，性别在伤口愈合中也起着至关重要的作用，男性和女性患者之间存在显著的微生物多样性。慢性溃疡患者在接受中药治疗（生肌膏）后取得了积极效果。从机制上讲，中药通过调节伤口微生物群来促进伤口愈合。
