G Sindhu, G L Shyni, Pushpan Chithra K, Nambisan Bala, A Helen
Department of Biochemistry, University of Kerala, Karyavattom, Thiruvananthapuram, 695581, India.
Central Tuber Crops Research Institute, Thiruvananthapuram, Kerala, India.
Prostaglandins Other Lipid Mediat. 2018 Sep;138:48-53. doi: 10.1016/j.prostaglandins.2018.08.002. Epub 2018 Aug 11.
Rheumatoid arthritis is a chronic inflammatory joint disease characterized by synovial proliferation and tissue destruction. Pro-inflammatory cytokines like interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) play a key role in the disease process and elevate energy expenditure, which further increases the joint pain and stiffness. The present study was undertaken to explore the anti-arthritic potential of fenugreek mucilage in adjuvant induced arthritic rats. In the present study, paw volume was measured on the 7, 14 and 21 day. Finally, animals were anaesthetized; blood samples and tissues were collected for the assay of inflammatory enzymes like cyclooxygenase, lipoxygenase; evaluated the level of cytokines like IL-6, TNF-α, arthritic index and rheumatoid factor. Fenugreek mucilage exhibited maximum percentage of edema inhibition at a dose of 75 mg/kg on 21st day of adjuvant arthritis. The effect was higher than that of standard drug, indomethacin. The activities of inflammatory enzymes and concentration of mediators were decreased on treatment with fenugreek mucilage. Cytology of synovial fluid showed mild inflammation with normal synoviocytes (mesothelial cells) tried to bring back to normal characteristics on supplementation with fenugreek mucilage. Based on the observations, it can be suggested that fenugreek mucilage possesses promising anti-arthritic property and it can be used as a therapeutic agent for arthritis.
类风湿性关节炎是一种慢性炎症性关节疾病，其特征为滑膜增生和组织破坏。白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）等促炎细胞因子在疾病过程中起关键作用，并增加能量消耗，这进一步加剧了关节疼痛和僵硬。本研究旨在探讨胡芦巴黏液对佐剂诱导的关节炎大鼠的抗关节炎潜力。在本研究中，于第7、14和21天测量爪体积。最后，将动物麻醉；采集血液样本和组织，用于检测环氧化酶、脂氧化酶等炎症酶；评估IL-6、TNF-α等细胞因子水平、关节炎指数和类风湿因子。在佐剂性关节炎第21天，胡芦巴黏液在剂量为75mg/kg时表现出最大程度的水肿抑制百分比。该效果高于标准药物吲哚美辛。用胡芦巴黏液治疗后，炎症酶活性和介质浓度降低。滑液细胞学显示轻度炎症，补充胡芦巴黏液后，正常的滑膜细胞（间皮细胞）试图恢复正常特征。基于这些观察结果，可以认为胡芦巴黏液具有有前景的抗关节炎特性，可作为关节炎的治疗药物。
