Sankar Sathish, Ramamurthy Mageshbabu, Suganya Subramanian, Nandagopal Balaji, Sridharan Gopalan
Sri Sakthi Amma Institute of Biomedical Research, Sri Narayani Hospital and Research Centre, Sripuram, Vellore - 632055, Tamil Nadu, India.
Bioinformation. 2018 May 31;14(5):183-189. doi: 10.6026/97320630014183. eCollection 2018.
Influenza viruses A and B are important human respiratory pathogens causing seasonal, endemic and pandemic infections in several parts of the globe with high morbidity and considerable mortality. The current inactivated and live attenuated vaccines are not effective. Therefore, it is of interest to design universal influenza virus vaccines with high efficacy. The peptide GQSVVSVKLAGNSSL of pandemic influenza, the peptide DKTSVTLAGNSSLCS of seasonal influenza and the peptide DILLKFSPTEITAPT of influenza B were identified as potential linear cell mediated epitopes. The epitopes predicted by BepiPred (B-cell epitope designer) program was subjected to docking experiment-using HexDock and CABS dock programs. The epitopes of pandemic H1N1 influenza A gave similar score of high affinity in docking. The epitope DKTSVTLAGNSSLCS of seasonal influenza A and epitope DILLKFSPTEITAPT of influenza B had high binding energy. It is further observed that the peptides GQSVVSVKLAGNSSL (pandemic influenza), DKTSVTLAGNSSLCS (seasonal influenza) DILLKFSPTEITAPT (influenza B) are found to interact with some known MHC class II alleles. These peptides have high-affinity binding with known MHC class II alleles. Thus, they have the potential to elicit cell immune response. These vaccines have to be further evaluated in animal models and human volunteers. These findings have application in the development of peptide B-cell epitope vaccines against influenza viruses.
甲型和乙型流感病毒是重要的人类呼吸道病原体，在全球多个地区引发季节性、地方性和大流行性感染，发病率高且死亡率可观。目前的灭活疫苗和减毒活疫苗效果不佳。因此，设计高效的通用流感病毒疫苗备受关注。大流行性流感的肽段GQSVVSVKLAGNSSL、季节性流感的肽段DKTSVTLAGNSSLCS以及乙型流感的肽段DILLKFSPTEITAPT被确定为潜在的线性细胞介导表位。通过BepiPred（B细胞表位设计器）程序预测的表位，使用HexDock和CABS dock程序进行对接实验。甲型大流行性H1N1流感的表位在对接中给出了相似的高亲和力分数。甲型季节性流感的表位DKTSVTLAGNSSLCS和乙型流感的表位DILLKFSPTEITAPT具有高结合能。进一步观察发现，肽段GQSVVSVKLAGNSSL（大流行性流感）、DKTSVTLAGNSSLCS（季节性流感）、DILLKFSPTEITAPT（乙型流感）与一些已知的II类主要组织相容性复合体（MHC）等位基因相互作用。这些肽段与已知的II类MHC等位基因具有高亲和力结合。因此，它们有引发细胞免疫反应的潜力。这些疫苗必须在动物模型和人类志愿者中进一步评估。这些发现可应用于开发针对流感病毒的肽段B细胞表位疫苗。
