Kidane Yosief, Bokrezion Temesgen, Mebrahtu Jimmy, Mehari Mikias, Gebreab Yacob Berhane, Fessehaye Nahom, Achila Oliver Okoth
Department of Clinical Laboratory Sciences, Asmara College of Health Sciences (ACHS), Eritrea.
Evid Based Complement Alternat Med. 2018 Jul 16;2018:2164345. doi: 10.1155/2018/2164345. eCollection 2018.
This research assessed the antidiabetic activity and phytochemical constituents of the traditionally used medicinal plants, .
The leaves of both plants were subjected to cold extraction method using 70% ethanol and hot Soxhlet extraction using n-hexane, chloroform, methanol, and distilled water. The extracts were studied for their effect on glucose transport across yeast cells and inhibition of -amylase and -glucosidase enzyme activities. Thin-layer chromatographic analysis of ethanol extract was also undertaken.
The results of yeast glucose uptake assay revealed that extracts from both plants had a maximum increase in glucose uptake at the 25mM glucose concentration with a maximum dose of 2000g/ml plant extract. The ethanol extract of and aqueous extract of showed a high activity of 68% and 96%, respectively, at 25mM and 2000g/ml of glucose and extract concentration. exerted peak inhibition activity of -amylase of 37.5 ± 3% mg/dl (IC = 0.523 mg/dl) for methanol and distilled water extract at 0.5 mg/dl, respectively. methanol extract exhibited the highest inhibition activity of 38 ± 8 % mg/dl (IC50 = 0.543 mg/dl) at 0.5 mg/dl. In the -glucosidase inhibition assay, the methanolic extract of exhibited the highest inhibitory activity of 17.29 ± 9% mg/dl (IC = 0.761 mg/dl) at 0.5mg/dl. The chloroform extract of had the highest inhibitory activity of 30 ± 5% mg/dl (IC = 0.6mg/dl) at 0.5 mg/dL. Phytochemical analysis of the different extracts of and revealed the presence of flavonoids, alkaloids, tannins, saponins, phytosterols, and carbohydrates. Thin-layer chromatography analysis of ethanolic extract of both plants indicated presence of 15 and 17 spots for and respectively.
and extracts have moderate inhibitory activity against pancreatic -amylase and relatively low inhibitory activities against -glucosidase. The observed effects may be associated with the presence of flavonoids, saponins, and alkaloids. Additional analysis, toxicological studies, isolation, and structural characterization of the phytomolecules identified in this study and molecular docking studies should be undertaken.
本研究评估了传统药用植物的抗糖尿病活性和植物化学成分。
两种植物的叶子分别采用70%乙醇冷提取法以及正己烷、氯仿、甲醇和蒸馏水热索氏提取法。研究了提取物对酵母细胞葡萄糖转运的影响以及对α-淀粉酶和α-葡萄糖苷酶活性的抑制作用。还对乙醇提取物进行了薄层色谱分析。
酵母葡萄糖摄取试验结果表明，两种植物的提取物在葡萄糖浓度为25mM、植物提取物最大剂量为2000μg/ml时，葡萄糖摄取量增加最多。在葡萄糖浓度为25mM、提取物浓度为2000μg/ml时，[植物1]的乙醇提取物和[植物2]的水提取物分别显示出68%和96%的高活性。[植物1]的甲醇提取物和蒸馏水提取物在0.5mg/ml时分别对α-淀粉酶表现出37.5±3%mg/dl的峰值抑制活性（IC50 = 0.523mg/dl）。[植物1]的甲醇提取物在0.5mg/ml时表现出最高抑制活性，为38±8%mg/dl（IC50 = 0.543mg/dl）。在α-葡萄糖苷酶抑制试验中，[植物1]的甲醇提取物在0.5mg/ml时表现出最高抑制活性，为17.29±9%mg/dl（IC50 = 0.761mg/dl）。[植物2]的氯仿提取物在0.5mg/dL时表现出最高抑制活性，为30±5%mg/dl（IC50 = 0.6mg/dl）。对[植物1]和[植物2]不同提取物的植物化学分析表明存在黄酮类、生物碱、单宁、皂苷、植物甾醇和碳水化合物。两种植物乙醇提取物的薄层色谱分析表明，[植物1]和[植物2]分别有15个和17个斑点。
[植物1]和[植物2]提取物对胰腺α-淀粉酶具有中等抑制活性，对α-葡萄糖苷酶的抑制活性相对较低。观察到的效果可能与黄酮类、皂苷和生物碱的存在有关。应进行本研究中鉴定的植物分子的进一步分析、毒理学研究、分离和结构表征以及分子对接研究。
