Shin Jaeha, Lee Sujin, Cha Misun
Biotechnology Institute , Medifab Co. LTD. , 70, Dusan-ro , Geumcheon-gu , Seoul , 08513 , South Korea . Email:
School of Mechanical and Aerospace Engineering , Seoul National University , 1 Gwanak-ro , Gwanak-gu , Seoul , Republic of Korea.
Medchemcomm. 2017 Feb 2;8(3):625-632. doi: 10.1039/c6md00716c. eCollection 2017 Mar 1.
Carbon nanotubes (CNTs) have emerged as a leading nanomaterial for biomedical applications because of their extraordinary properties, which make them useful as delivery vehicles for drugs, proteins, and DNA into cells. However, the numerous applications of carbon nanotubes inevitably increase the potential risk of this nanomaterial. To address this issue, it is necessary to develop protocols for the effective and safe degradation of CNTs. In this study, we demonstrate a self-degradation route for single-wall carbon nanotubes mediated by the built-in peroxidase-like activity of bacterial magnetic nanoparticles (BMPs). Biocompatible BMPs which originated from sp. AMB-1 were directly conjugated through covalent bonding to functionalized SWNTs (f-SWNTs) without any additional functionalization processes. This SWNT-BMP hybrid was proven to exhibit highly synergetic peroxidase-like activity, and BMPs act as a highly effective intrinsic peroxidase for the self-degradation of BMP-decorated SWNTs. Moreover, it was shown to be an inhibitor that reduces the formation of β-amyloid (Aβ) fibrils, which are considered a key element in Alzheimer's disease. Thereby the SWNT-BMP hybrid exerts neuroprotective effects against β-amyloid (Aβ) fibrillation-induced neurotoxicity in SH-SY5Y human neuroblastoma cells. These results suggest that the SWNT-BMP hybrid could offer a new approach for treating or preventing neurodegenerative diseases.
碳纳米管（CNTs）因其非凡的特性，已成为生物医学应用领域的一种领先纳米材料，这些特性使其作为药物、蛋白质和DNA进入细胞的递送载体十分有用。然而，碳纳米管的众多应用不可避免地增加了这种纳米材料的潜在风险。为解决这一问题，有必要开发有效且安全降解碳纳米管的方案。在本研究中，我们展示了一种由细菌磁性纳米颗粒（BMPs）的内在类过氧化物酶活性介导的单壁碳纳米管自降解途径。源自AMB-1菌的生物相容性BMPs通过共价键直接与功能化单壁碳纳米管（f-SWNTs）共轭，无需任何额外的功能化过程。这种单壁碳纳米管-细菌磁性纳米颗粒杂化物被证明具有高度协同的类过氧化物酶活性，并且细菌磁性纳米颗粒作为一种高效的内在过氧化物酶，用于降解经细菌磁性纳米颗粒修饰的单壁碳纳米管。此外，它被证明是一种抑制剂，可减少β-淀粉样蛋白（Aβ）纤维的形成，而β-淀粉样蛋白纤维被认为是阿尔茨海默病的关键因素。因此，单壁碳纳米管-细菌磁性纳米颗粒杂化物对SH-SY5Y人神经母细胞瘤细胞中β-淀粉样蛋白（Aβ）纤维化诱导的神经毒性具有神经保护作用。这些结果表明，单壁碳纳米管-细菌磁性纳米颗粒杂化物可为治疗或预防神经退行性疾病提供一种新方法。
