Key Laboratory of Analytical Chemistry for Life Science (MOE), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
Chemistry. 2011 Aug 16;17(34):9377-84. doi: 10.1002/chem.201100478. Epub 2011 Jul 18.
A combined hydrothermal/hydrogen reduction method has been developed for the mass production of helical carbon nanotubes (HCNTs) by the pyrolysis of acetylene at 475 °C in the presence of Fe(3)O(4) nanoparticles. The synthesized HCNTs have been characterized by high-resolution transmission electron microscopy, scanning electron microscopy, X-ray diffraction analysis, vibrating sample magnetometry, and contact-angle measurements. The as-prepared helical-structured carbon nanotubes have a large specific surface area and high peroxidase-like activity. Catalysis was found to follow Michaelis-Menten kinetics and the HCNTs showed strong affinity for both H(2)O(2) and 3,3',5,5',-tetramethylbenzidine (TMB). Based on the high activity, the HCNTs were firstly used to develop a biocatalyst and amperometric sensor. At pH 7.0, the constructed amperometric sensor showed a linear range for the detection of H(2)O(2) from 0.5 to 115 μM with a correlation coefficient of 0.999 without the need for an electron-transfer mediator. Because of their low cost and high stability, these novel metallic HCNTs represent a promising candidate as mimetic enzymes and may find a wide range of new applications, such as in biocatalysis, immunoassay, and environmental monitoring.
一种联合水热/氢气还原法已经被开发出来，用于通过在 475°C 下，在 Fe(3)O(4)纳米粒子存在下，使乙炔热解来大规模生产螺旋状碳纳米管 (HCNTs)。通过高分辨率透射电子显微镜、扫描电子显微镜、X 射线衍射分析、振动样品磁强计和接触角测量对合成的 HCNTs 进行了表征。所制备的螺旋结构碳纳米管具有大的比表面积和高的过氧化物酶样活性。发现催化遵循米氏动力学，并且 HCNTs 对 H(2)O(2)和 3,3',5,5',-四甲基联苯胺 (TMB)都表现出强烈的亲和力。基于高活性，首次将 HCNTs 用于开发生物催化剂和安培传感器。在 pH 7.0 下，构建的安培传感器在检测 H(2)O(2)时显示出从 0.5 到 115 μM 的线性范围，相关系数为 0.999，而无需电子转移介质。由于其低成本和高稳定性，这些新型金属 HCNTs 作为模拟酶具有很大的应用前景，并可能在生物催化、免疫分析和环境监测等领域找到广泛的新应用。
