Costa Juliana de Oliveira, Resende José Adão, Gil Frederico Ferreira, Santos Joseph Fabiano Guimarães, Gomes Maria Aparecida
MPH, PharmD. Pharmacist, Department of Social and Preventive Medicine, Postgraduate Program in Public Health, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte (MG), Brazil.
Clinical analysis technician, Hermes Pardini Laboratory, Belo Horizonte (MG), Brazil.
Sao Paulo Med J. 2018 Jul-Aug;136(4):319-323. doi: 10.1590/1516-3180.2018.0036170418. Epub 2018 Aug 13.
Enteral parasitic diseases are a public health problem in nations with low economic development and in settings with poor sanitation. Amebiasis is the second most frequent form of parasitosis, with a high burden of disease. Knowledge of the prevalence of enteroparasitoses in a given region is useful for planning clinical decision-making. Thus, the aim of this study was to estimate the prevalence of enteral parasitic diseases, especially amebiasis, through analysis on stool samples from public and private laboratories in a metropolitan area in southeastern Brazil.
Cross-sectional study conducted in the metropolitan region of Belo Horizonte, Brazil.
We evaluated 6,289 fecal samples from one private and one public laboratory. The samples were concentrated by means of spontaneous sedimentation, and those that were positive for Entamoeba histolytica or Entamoeba dispar in optical microscopy analyses were processed to obtain deoxyribonucleic acid, with subsequent identification through the polymerase chain reaction.
Among the stool samples, 942 (15.0%) had parasitic infections; 73 (1.2%) of these were helminthic infections and 847 (13.5%) were protozoan infections, caused mainly by Escherichia coli (6.0%), Endolimax nana (5.2%) and Giardia lamblia (1.2%). Infections due to Entamoeba histolytica or Entamoeba dispar occurred in 36 samples (0.6%) and the polymerase chain reaction revealed five (13.9%) as Entamoeba histolytica.
The prevalence of enteral parasitic diseases is high in the metropolitan region of Belo Horizonte, although amebiasis may not be a problem.
肠道寄生虫病在经济发展水平较低且卫生条件较差的国家是一个公共卫生问题。阿米巴病是第二常见的寄生虫病形式，疾病负担较重。了解特定地区肠道寄生虫病的流行情况有助于规划临床决策。因此，本研究的目的是通过对巴西东南部一个大都市地区公立和私立实验室的粪便样本进行分析，估计肠道寄生虫病尤其是阿米巴病的流行情况。
在巴西贝洛奥里藏特市大都市区进行的横断面研究。
我们评估了来自一个私立实验室和一个公立实验室的6289份粪便样本。样本通过自然沉淀法浓缩，在光学显微镜分析中对溶组织内阿米巴或迪斯帕内阿米巴呈阳性的样本进行处理以获取脱氧核糖核酸，随后通过聚合酶链反应进行鉴定。
在粪便样本中，942份（15.0%）有寄生虫感染；其中73份（1.2%）为蠕虫感染，847份（13.5%）为原生动物感染，主要由大肠埃希菌（6.0%）、微小内蜒阿米巴（5.2%）和蓝氏贾第鞭毛虫（1.2%）引起。溶组织内阿米巴或迪斯帕内阿米巴感染发生在36份样本中（0.6%），聚合酶链反应显示其中5份（13.9%）为溶组织内阿米巴。
贝洛奥里藏特市大都市区肠道寄生虫病的流行率较高，尽管阿米巴病可能不是一个问题。
