Ma Wei-Si, Chen Jun, Yan Shi-Wu, Wang Xin, Dong Zhi-Yuan, Li Lin-Yu, Yang Li-Ying, Yang Bin
Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650205, China.
Institute of Medicinal Plant Development， Chinese Academy of Medical Science and Peking Union Medical College， Beijing 100193， China.
Zhongguo Zhong Yao Za Zhi. 2018 Jul;43(14):2918-2927. doi: 10.19540/j.cnki.cjcmm.20180510.003.
Gray mold disease is one of the most important diseases of planted Paris polyphylla var. yunnanensis, the disease appeared primarily as blossom blights and fruit rots, but also as stem rots, leaf rots.In this study, the pathogenetic fungi was isolated from plant tissue or sclerotia that covering the fruit of diseased P. polyphylla var. yunnanensis, the pathogen was certified according to Koch's Postulation. The pathogen produced abundant black, irregular sclerotia on surface of diseased plants and potato dextrose agar. The conidiophores and clusters of oval conidia resembled a grape-like cluster, the size of conidia was 9.70-13.70 μm [average of (11.32±0.82)μm]×7.05-9.12 μm [average of (8.24±0.48)μm], the microconidia produced on potato dextrose agar were spherical,and the size was （3.34±0.31） μm,the pathogen was identified as Botrytis sp based on morphological characteristics. The DNA sequence analysis of the G3PDH, HSP60, RPB2 genes placed the pathogen in a single clade that outside defined species of Botrytis, so the pathogen could be identified as a new species of Botrytis. The pathogen requires 20 °C, pH 8, darkness or low light condition for the best growth.
灰霉病是滇重楼种植中最重要的病害之一，该病主要表现为花腐和果腐，也有茎腐和叶腐。本研究从染病滇重楼果实上覆盖的植物组织或菌核中分离出致病真菌，并根据柯赫氏法则对病原菌进行了鉴定。病原菌在染病植株表面和马铃薯葡萄糖琼脂培养基上产生大量黑色、不规则的菌核。分生孢子梗和椭圆形分生孢子簇形似葡萄串，分生孢子大小为9.70 - 13.70μm[平均(11.32±0.82)μm]×7.05 - 9.12μm[平均(8.24±0.48)μm]，在马铃薯葡萄糖琼脂培养基上产生的小分生孢子为球形，大小为(3.34±0.31)μm，根据形态特征将病原菌鉴定为葡萄孢属真菌。对G3PDH、HSP60、RPB2基因的DNA序列分析表明，该病原菌位于葡萄孢属已定义物种之外的一个单分支中，因此该病原菌可被鉴定为葡萄孢属的一个新物种。病原菌在20℃、pH 8、黑暗或弱光条件下生长最佳。
