Li L-H, Lv M-M, Li X, Ye T-Z, He X, Rong S-H, Dong Y-L, Guan Y, Gao X-L, Zhu J-Q, Xu Z-J
Rice Institute of Sichuan Agricultural University, Key Laboratory of Southwest Crop Genetic Resources and Improvement, Ministry of Education, Chengdu, 611130 China.
Mol Biol (Mosk). 2018 Jul-Aug;52(4):567-575. doi: 10.1134/S0026898418040122.
With the advance of sequencing technology, the number of sequenced plant genomes has been rapidly increasing. However, understanding of the gene function in these sequenced genomes lags far behind; as a result, many coding plant sequences in public databases are annotated as proteins with domains of unknown function (DUF). Function of a protein family DUF810 in rice is not known. In this study, we analysed seven members of OsDU810 (OsDUF810.1-OsDUF810.7) family with three distinct motifs in rice Nipponbare. By phylogenetic analysis, OsDUF810 proteins fall into three major groups (I, II, III). Expression patterns of the seven corresponding OsDUF810 protein-encoding genes in 15 different rice tissues vary. Under drought, salt, cold and heat stress conditions and ABA treatment, the expression of OsDUF810.7 significantly increases. Overexpression of this protein in E. coli lead to a significant enhancement of catalase (CAT) and peroxidase (POD) activities, and improved bacterial resistance to salt and drought.
随着测序技术的进步，已测序的植物基因组数量迅速增加。然而，对这些已测序基因组中基因功能的了解却远远滞后；因此，公共数据库中许多编码植物的序列被注释为具有未知功能结构域（DUF）的蛋白质。水稻中蛋白质家族DUF810的功能尚不清楚。在本研究中，我们分析了日本晴水稻中具有三个不同基序的OsDU810（OsDUF810.1 - OsDUF810.7）家族的七个成员。通过系统发育分析，OsDUF810蛋白分为三个主要组（I、II、III）。七个相应的OsDUF810蛋白编码基因在15种不同水稻组织中的表达模式各不相同。在干旱、盐、冷和热胁迫条件以及ABA处理下，OsDUF810.7的表达显著增加。在大肠杆菌中过表达该蛋白导致过氧化氢酶（CAT）和过氧化物酶（POD）活性显著增强，并提高了细菌对盐和干旱的抗性。
