Faculty of Pharmaceutical Sciences, School of Health Sciences, University of Iceland, Iceland; Biomedical Center, University of Iceland, Iceland; ArcticMass, Reykjavk, Iceland.
Center for Systems Biology, University of Iceland, Iceland; Biomedical Center, University of Iceland, Iceland.
Int J Biochem Cell Biol. 2018 Oct;103:99-104. doi: 10.1016/j.biocel.2018.08.003. Epub 2018 Aug 13.
Epithelial to mesenchymal transition (EMT) is a developmental event characterized by phenotypic switching from a polarized epithelial phenotype to an unpolarized mesenchymal phenotype. Changes to plasma membrane function accompany EMT yet the differences in lipid composition of cells that have undergone EMT are relatively unexplored. To address this the lipidome of two cell models of EMT in breast epithelial tissue, D492 and HMLE, were analyzed by untargeted LC-MS. Detected masses were identified and their abundance was compared through multivariate statistical analysis. Considerable concordance was observed in eight lipid components between epithelial and mesenchymal cells in both cell models. Specifically, an increase in phosphatidylcholine and triacylglycerol were found to accompany EMT while phosphatidylcholine- and phosphatidylethanolamine plasmalogens, as well as diacylglycerols decreased. The most abundant fatty acid lengths were C16 and C18 but mesenchymal cells had on average shorter and more unsaturated fatty acids. The results are consistent with enhanced cell mobility post EMT and reflect a consequence of oxidative stress pre- and post EMT in breast epithelial tissue.
上皮间质转化（EMT）是一种发育事件，其特征是表型从极化的上皮表型转变为非极化的间质表型。尽管伴随 EMT 发生了质膜功能的变化，但 EMT 细胞的脂质组成差异尚未得到充分探索。为了解决这一问题，我们通过非靶向 LC-MS 分析了乳腺上皮组织中两种 EMT 细胞模型（D492 和 HMLE）的脂质组。鉴定了检测到的物质，并通过多变量统计分析比较了它们的丰度。在这两种细胞模型中，上皮细胞和间充质细胞之间有八个脂质成分具有相当大的一致性。具体而言，发现 EMT 时伴随着磷脂酰胆碱和三酰甘油的增加，而磷脂酰胆碱和磷脂酰乙醇胺质体，以及二酰基甘油减少。最丰富的脂肪酸长度为 C16 和 C18，但间充质细胞的脂肪酸平均较短且不饱和程度更高。这些结果与 EMT 后细胞迁移能力增强一致，反映了 EMT 前和 EMT 后乳腺上皮组织中氧化应激的结果。
