Department of Nutrition and Dietetics, Harokopio University, 70 Eleftheriou Venizelou Avenue Kallithea, 17671, Athens, Greece.
Lipids Health Dis. 2018 Aug 16;17(1):187. doi: 10.1186/s12944-018-0836-z.
Excessive oxidative stress may impair bio-molecules and cellular function. Multi antioxidant supplementation is thought to be more effective than a single antioxidant probably through the synergistic or complementary action of natural substances that could enhance the prospective effect.
In order to estimate the effect of a plant extract based supplement in apparently healthy volunteers' oxidative stress markers, a double-blind and placebo controlled intervention was performed. 62 apparently healthy volunteers, overweight with medium adherence to the Mediterranean diet, were recruited and randomly allocated into two intervention groups (supplement or placebo) for 8 weeks. Basic biochemical markers, oxidized LDL (oxLDL), resistance of serum in oxidation, protein carbonyls in serum and 8-isoprostane and DNA/RNA damage in urine were measured.
No differentiation was observed in basic biochemical markers, in oxLDL levels as well as in serum resistance against oxidation, during intervention in the examined groups. A significant resistance regarding urine isoprostanes levels in the supplement group compared to the placebo one, was observed. Reduction on DNA/RNA damage and on protein carbonyls levels (almost 30% and 20% respectively, at 8 weeks) was detected in volunteers who consumed the supplement compared to the control group.
Consumption of plant extract based supplement seems to reduce DNA/RNA and protein oxidation and in less extent lipids peroxidation.
ClinicalTrials.gov Identifier for this study is: NCT02837107.
过度的氧化应激可能会损害生物分子和细胞功能。多抗氧化剂补充剂被认为比单一抗氧化剂更有效，可能是通过天然物质的协同或互补作用，从而增强预期效果。
为了评估一种基于植物提取物的补充剂对明显健康志愿者氧化应激标志物的影响，进行了一项双盲和安慰剂对照干预研究。招募了 62 名明显健康、超重且对地中海饮食有中等依从性的志愿者，并将他们随机分配到两个干预组（补充剂或安慰剂）进行 8 周的干预。测量了基本生化标志物、氧化型 LDL（oxLDL）、血清氧化抵抗力、血清蛋白羰基和 8-异前列腺素以及尿液中的 DNA/RNA 损伤。
在接受干预的两组中，基本生化标志物、oxLDL 水平以及血清氧化抵抗力均未观察到差异。与安慰剂组相比，补充剂组尿液中异前列腺素水平的抵抗力显著增强。与对照组相比，服用补充剂的志愿者的 DNA/RNA 损伤和蛋白羰基水平（分别降低了 30%和 20%，在 8 周时）有所降低。
食用基于植物提取物的补充剂似乎可以减少 DNA/RNA 和蛋白质氧化，以及脂质过氧化的程度较小。
本研究的 ClinicalTrials.gov 标识符为：NCT02837107。
