新型化合物 XH-103 对辐射诱导的胃肠道综合征的保护作用。
The Protective Effect of New Compound XH-103 on Radiation-Induced GI Syndrome.
机构信息
Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China.
出版信息
Oxid Med Cell Longev. 2018 Jul 4;2018:3920147. doi: 10.1155/2018/3920147. eCollection 2018.
BACKGROUND
Radiation-induced intestinal injury is one of the side effects in patients receiving radiotherapy. The aim of the present study was to investigate the protective effect of XH-103 on radiation-induced small intestinal injury and to explore its mechanism.
METHODS
C57BL/6N mice were irradiated and treated with XH-103. Firstly, the survival rate of mice exposed to 9.0 Gy and 11.0 Gy total body irradiation (TBI) was examined. Subsequently, at 3.5 d after IR, the small intestinal morphological changes were examined by HE. The numbers of crypt cells, the villus height, the expression of Ki67 and Lgr5, and the apoptotic cells in the intestinal crypts were examined by immunohistochemistry. Furthermore, the expression of p53 and Bax was analyzed by WB.
RESULTS
Compared to the irradiation group, XH-103 improved the mice survival rate, protected the intestinal morphology of mice, decreased the apoptotic rate of intestinal crypt cells, maintained cell regeneration, and promoted crypt proliferation and differentiation. XH-103 also reduced the expression of p53 and Bax in the small intestine compared to the IR group.
CONCLUSION
These data demonstrate that XH-103 can prevent radiation-induced intestinal injury, which is beneficial for the protection of radiation injuries.
背景
放射性肠损伤是接受放射治疗的患者的一种副作用。本研究旨在探讨 XH-103 对放射性小肠损伤的保护作用及其机制。
方法
C57BL/6N 小鼠接受 XH-103 照射和处理。首先，检测暴露于 9.0Gy 和 11.0Gy 全身照射（TBI）的小鼠的存活率。随后，在 IR 后 3.5d，通过 HE 观察小肠形态变化。通过免疫组织化学检测隐窝细胞数量、绒毛高度、Ki67 和 Lgr5 的表达以及肠隐窝中的凋亡细胞。此外，通过 WB 分析 p53 和 Bax 的表达。
结果
与照射组相比，XH-103 提高了小鼠的存活率，保护了小鼠的肠道形态，降低了肠隐窝细胞的凋亡率，维持了细胞再生，并促进了隐窝增殖和分化。与 IR 组相比，XH-103 还降低了小肠中 p53 和 Bax 的表达。
结论
这些数据表明，XH-103 可以预防放射性肠损伤，有利于保护放射性损伤。
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