利用异种杂交瘤生产具有抗-D（恒河猴）特异性的人单克隆IgG和IgM抗体。
Production of human monoclonal IgG and IgM antibodies with anti-D (rhesus) specificity using heterohybridomas.
作者信息
Thompson K M, Melamed M D, Eagle K, Gorick B D, Gibson T, Holburn A M, Hughes-Jones N C
出版信息
Immunology. 1986 May;58(1):157-60.
Abstract
Heterohybridomas secreting human IgM and IgG anti-D antibodies of the rhesus blood group system have been established by fusion of EBV-transformed anti-D secreting cells with the mouse myeloma cells X63-Ag8.653. Both classes of antibody reacted with all Rh-positive cells, some Du cells but not with Rh-negative or DB cells. Concentrations of both antibodies reached between 25 micrograms/ml and 50 micrograms/ml in the culture supernatants. The cell lines have been maintained in culture for 14 months and have been shown to be suitable for large-scale production of antibody.
摘要
通过将EB病毒转化的分泌抗-D抗体的细胞与小鼠骨髓瘤细胞X63-Ag8.653融合，已建立了分泌恒河猴血型系统的人IgM和IgG抗-D抗体的异种杂交瘤。这两类抗体都与所有Rh阳性细胞、一些Du细胞发生反应，但不与Rh阴性或DB细胞发生反应。培养上清液中两种抗体的浓度达到25微克/毫升至50微克/毫升之间。这些细胞系已在培养中维持了14个月，并已证明适用于大规模生产抗体。
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