Jiangsu Province Engineering Research Center for Marine Bio-Resources Sustainable Utilization and College of Oceanography, Hohai University, Nanjing, People's Republic of China.
Guangdong Provincial Key Laboratory of Plant Stress Biology and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, People's Republic of China.
Antonie Van Leeuwenhoek. 2024 Oct 29;118(1):24. doi: 10.1007/s10482-024-02034-z.
Strain WL0113 was isolated from surface seawater of the coast of Lianyungang, Jiangsu province, PR China. Strain WL0113 shared highest 16S rRNA gene sequence similarity with Roseobacter insulae YSTF-M11 (98.8%), followed by R. cerasinus AI77 (98.8%), R. ponti MM-7 (98.0%). Strain WL0113 was Gram-stain-negative, cream, aerobic, non-motile and coccoid- to oval-shaped, and able to grow at pH 6.5-9.0 (optimum, pH 7.0-8.0), at 10-37 °C (optimum, 28 °C) and in the presence of 1-5% (w/v; optimum, 2.5%) NaCl. Ubiquinone-10 was detected as dominant. The main fatty acids (> 5%) of the strain WL0113 were C, iso-C 3OH, Cω6,9,12,15c (arachidonic acid), and summed feature 8 (Cω7c and/or Cω6c). The major polar lipids include phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, glycophospholipid, unknown aminolipid, unknown phospholipid, and two unknown polar lipids. The ANI and dDDH values between strain WL0113 and Roseobacter cerasinus were 80.4% and 23.0%, respectively. The genomic DNA G + C content of strain WL0113 was 63.1%. Based on these data, it is proposed that strain WL0113 represent novel species of the genus Roseobacter, for which the name Roseobacter sinensis sp. nov. is proposed. The type strain is WL0113 (= GDMCC 1.3082 = JCM 35567).
菌株 WL0113 从中国江苏省连云港市沿海的海水表面分离得到。菌株 WL0113 的 16S rRNA 基因序列与 Roseobacter insulae YSTF-M11（98.8%）最为相似，其次是 R. cerasinus AI77（98.8%）和 R. ponti MM-7（98.0%）。菌株 WL0113 革兰氏染色阴性，奶油色，需氧，不运动，呈球形到卵形，能够在 pH 值 6.5-9.0（最佳，pH 值 7.0-8.0）、10-37°C（最佳，28°C）和 1-5%（w/v；最佳，2.5%）NaCl 存在的条件下生长。检测到的主要醌类为 ubiquinone-10。菌株 WL0113 的主要脂肪酸（>5%）为 C、iso-C 3OH、Cω6,9,12,15c（花生四烯酸）和 summed feature 8（Cω7c 和/或 Cω6c）。主要极性脂包括磷脂酰甘油、双磷脂酰甘油、磷脂酰胆碱、糖脂、未知胺脂、未知磷脂和两种未知极性脂。菌株 WL0113 与 Roseobacter cerasinus 的 ANI 和 dDDH 值分别为 80.4%和 23.0%。菌株 WL0113 的基因组 DNA G+C 含量为 63.1%。基于这些数据，建议菌株 WL0113 代表 Roseobacter 属的一个新种，命名为 Roseobacter sinensis sp. nov.。模式菌株为 WL0113（=GDMCC 1.3082=JCM 35567）。
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