Alattar Nebras, Daud Hasbullah, Al-Majmaie Rasoul, Zeulla Domonic, Al-Rubeai Mohameed, Rice James H
Appl Opt. 2018 Aug 1;57(22):E184-E189. doi: 10.1364/AO.57.00E184.
Raman-spectroscopy-based methods, such as surface-enhanced Raman spectroscopy, are a well-evolved method to molecular fingerprint cell types. Here we demonstrate that surface-enhanced Raman spectroscopy can enable us to distinguish cell development stages of bone marrow hematopoietic stem cells towards red blood cells through the identification of specific surface-enhanced Raman spectroscopy biomarkers. The approach taken here is to allow cells to take in gold nanoparticles as Raman enhancement platforms for kinetic structural observations presented here through the view of the multidimensional parameter contribution, thereby enabling profiling of bone marrow hematopoietic stem cells acquired from proliferation (stage one), differentiation (stage two), and mature red blood cells (stage three).
基于拉曼光谱的方法，如表面增强拉曼光谱，是一种用于分子指纹识别细胞类型的成熟方法。在这里，我们证明表面增强拉曼光谱能够使我们通过识别特定的表面增强拉曼光谱生物标志物，区分骨髓造血干细胞向红细胞的细胞发育阶段。这里采用的方法是让细胞摄取金纳米颗粒作为拉曼增强平台，通过多维参数贡献的视角进行此处呈现的动力学结构观察，从而能够对从增殖阶段（第一阶段）、分化阶段（第二阶段）和成熟红细胞阶段（第三阶段）获取的骨髓造血干细胞进行分析。
