Cui Zihong, Xu Zhiwei, Wei Yunlin, Zhang Qi, Qin Kunhao, Ji Xiuling
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China.
Curr Microbiol. 2021 Jan;78(1):150-158. doi: 10.1007/s00284-020-02277-9. Epub 2020 Nov 4.
Although bacteriophages are more numerous and have smaller genomes than their bacterial hosts, relatively few have their genomes sequenced. Here, we isolated the Pseudomonas fluorescens bacteriophage from Napahai plateau wetland and performed de novo genome sequencing. Based on the previous biological characteristics and bioinformatics analysis, it was determined that VW-6B was a linear double-stranded DNA (dsDNA) phage with 35,306 bp, with 56.76% G+C content and 197 bp tandem repeats. The VW-6B genome contained 46 open-reading frames (ORFs), and no tRNA genes were found. Based on phage genome structure, sequence comparison, and collinear analysis, VW-6B should be classified into the family Siphoviridae and be considered as a member of a new species in the Mu-like phage. The newly isolated bacteriophage can specifically infect P. fluorescens, which further enriches the diversity of known bacteriophages and provides a basis for the subsequent research and application of bacteriophages.
尽管噬菌体比其细菌宿主数量更多且基因组更小，但相对而言只有少数噬菌体的基因组被测序。在此，我们从纳帕海高原湿地分离出荧光假单胞菌噬菌体，并进行了从头基因组测序。基于先前的生物学特性和生物信息学分析，确定VW-6B是一种线性双链DNA（dsDNA）噬菌体，大小为35306 bp，G+C含量为56.76%，具有197 bp的串联重复序列。VW-6B基因组包含46个开放阅读框（ORF），未发现tRNA基因。基于噬菌体基因组结构、序列比较和共线性分析，VW-6B应归类于长尾噬菌体科，被认为是类Mu噬菌体中新物种的一个成员。新分离的噬菌体能够特异性感染荧光假单胞菌，这进一步丰富了已知噬菌体的多样性，并为后续噬菌体的研究和应用提供了依据。
