从复杂生物样本中生成感染性RNA病毒群落的方案

Protocol for Generating Infectious RNA Viromes from Complex Biological Samples.

作者信息

Monteil-Bouchard Sonia, Temmam Sarah, Desnues Christelle

机构信息

Unité de Recherche sur les Maladies Infectieuses Tropicales Emergentes (URMITE), IHU Méditerranée Infection, Assistance-Publique des Hôpitaux de Marseille, Aix-Marseille Université, CNRS 7278, IRD 198, INSERM1095, Marseille, France.

Biology of Infection Unit, Laboratory of Pathogen Discovery, Institut Pasteur, INSERM U1117, Paris, France.

出版信息

Methods Mol Biol. 2018;1838:25-36. doi: 10.1007/978-1-4939-8682-8_3.

DOI:10.1007/978-1-4939-8682-8_3

PMID:30128987

Abstract

This chapter proposes a simple, standardized protocol for generating RNA viromes from complex host-associated biological samples of various origins. Compared to other existing protocols to generate RNA viromes, this protocol preserves the infectivity of viral particles and allows for downstream applications such as viral characterization and isolation tests.

摘要

本章提出了一种简单、标准化的方案，用于从各种来源的复杂宿主相关生物样本中生成RNA病毒群落。与其他现有的生成RNA病毒群落的方案相比，该方案保留了病毒颗粒的感染性，并允许进行下游应用，如病毒鉴定和分离测试。