sp. nov.，分离自土壤。

sp. nov., Isolated from Soil.

机构信息

Department of Biological Science, College of Science and Engineering, Sangji University, Wonju 26339, Republic of Korea.

Department of Microbiology, Pukyong National University, Busan 48513, Republic of Korea.

出版信息

J Microbiol Biotechnol. 2024 Aug 28;34(8):1636-1641. doi: 10.4014/jmb.2404.04038. Epub 2024 Jun 17.

DOI:10.4014/jmb.2404.04038

PMID:39086223

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11380503/

Abstract

A Gram-stain-positive, aerobic, white-coloured, rod-shaped bacteria, designated as a strain dW9, was isolated from soil. Strain dW9 was catalase-positive and oxidase-negative. Strain dW9 grew at temperature of 20-37°C and at pH of 5.0-7.0. Phylogenetic and 16S rRNA gene analysis indicated that strain dW9 belonged to the genus with its closest relative being S4 (97.4% sequence similarity). The genome size of dW9 was 7,787,916 bp with DNA G+C content of 51.3%. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values of dW9 with its closest relatives were found to be <22.0% and <74.0%, respectively. The only respiratory quinone was MK-7, and the major fatty acids were antiso-C and iso-C. Overall, the comprehensive taxonomic analysis revealed that strain dW9 met all the fundamental criteria to be classified as a novel species within the genus Paenibacillus. Accordingly, we propose the name sp. nov., with the type strain dW9 (=KCTC 43431 =NBRC 116022).

摘要

一株革兰氏阳性、需氧、白色、杆状细菌，被命名为菌株 dW9，从土壤中分离得到。菌株 dW9 过氧化氢酶阳性，氧化酶阴性。菌株 dW9 在 20-37°C 的温度和 pH5.0-7.0 的条件下生长。系统发育和 16S rRNA 基因分析表明，菌株 dW9 属于属，与最接近的种为 S4（序列相似性为 97.4%）。dW9 的基因组大小为 7,787,916 bp，GC 含量为 51.3%。dW9 与最接近的种的数字 DNA-DNA 杂交（dDDH）和平均核苷酸同一性（ANI）值分别为<22.0%和<74.0%。唯一的呼吸醌是 MK-7，主要脂肪酸是anteiso-C 和 iso-C。总的来说，综合分类分析表明，菌株 dW9 符合所有分类学标准，可被归类为属中的一个新种。因此，我们建议使用菌株 dW9（=KCTC 43431 =NBRC 116022）作为 Paenibacillus 种的新种。