Kim Dong-Uk, Kim Ju-Young, Jang Jun Hwee, Kim Myung Kyum
Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Oct;68(10):3184-3189. doi: 10.1099/ijsem.0.002964. Epub 2018 Aug 21.
A bacterial strain, designated 17SD1-15, was isolated from soil. Cells of this strain were Gram-stain-negative and aerobic rods. The major fatty acids of strain 17SD1-15 were iso-C15 : 0 and summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I). The polar lipids were phosphatidylethanolamine, one phospholipid and five unidentified lipids. The G+C content of the genomic DNA of strain 17SD1-15 was 49.0 mol%. The 16S rRNA gene sequence analysis showed that strain 17SD1-15 was phylogenetically related to Pontibacter saemangeumensis GCM0142, Pontibacter korlensis X14-1, Pontibacter yuliensis H9X, Pontibacter diazotrophicus H4X and Pontibacter humi SWU8 (98.3, 96.4, 96.4, 96.4 and 96.0 % sequence similarity, respectively). DNA-DNA relatedness between 17SD1-15 and the most closely related type strain of Pontibacter species was 42.9±0.8 %. The low level of DNA-DNA relatedness identified strain 17SD1-15 as a member of a novel species in the genus Pontibacter. The results of genotypic and phenotypic data, including chemotaxonomic traits, showed that strain 17SD1-15 could be distinguished from its phylogenetically related species. Therefore, strain 17SD1-15 represents a novel species within the genus Pontibacter, for which the name Pontibacter terrae sp. nov. is proposed, with the type strain 17SD1-15 (=KCTC 52915=NBRC 113057).
从土壤中分离出一株名为17SD1-15的细菌菌株。该菌株细胞革兰氏染色阴性，为需氧杆菌。菌株17SD1-15的主要脂肪酸为异-C15 : 0和特征性总和4（anteiso-C17 : 1 B和/或异-C17 : 1 I）。极性脂质为磷脂酰乙醇胺、一种磷脂和五种未鉴定的脂质。菌株17SD1-15基因组DNA的G+C含量为49.0 mol%。16S rRNA基因序列分析表明，菌株17SD1-15在系统发育上与沙门氏菌Pontibacter saemangeumensis GCM0142、Pontibacter korlensis X14-1、Pontibacter yuliensis H9X、Pontibacter diazotrophicus H4X和Pontibacter humi SWU8相关（序列相似性分别为98.3%、96.4%、96.4%、96.4%和96.0%）。17SD1-15与Pontibacter属最密切相关的模式菌株之间的DNA-DNA相关性为42.9±0.8%。DNA-DNA相关性水平较低，确定菌株17SD1-15为Pontibacter属的一个新物种成员。基因型和表型数据（包括化学分类特征）的结果表明，菌株17SD1-15可与其系统发育相关物种区分开来。因此，菌株17SD1-15代表Pontibacter属内的一个新物种，为此提出名为Pontibacter terrae sp. nov.的新名称，模式菌株为17SD1-15（=KCTC 52915=NBRC 113057）。
