Pittaluga S, Raffeld M, Lipford E H, Cossman J
Blood. 1986 Jul;68(1):134-9.
The phenotypes of early stages of T cell maturation are reflected by precursor T (lymphoblastic) neoplasms. In the present study, a series of such neoplasms was analyzed to reveal the developmental association of the expression of stage-related cell surface markers and T cell receptor gene rearrangement. Rearrangements of the T cell receptor beta-chain (T beta) gene were found in most, but not all, cases (88%) of T cell lymphoblastic neoplasms. T beta gene rearrangement preceded surface expression of the T cell receptor-linked molecular complex T3. Of all monoclonal anti-T cell antibodies tested, only antibody 3A1 was capable of reacting with neoplastic cells from all cases irrespective of the occurrence of T cell receptor gene rearrangements. In contrast, markers T1 and T11, normally expressed by mature T cells, were absent from the neoplastic cells in many cases (73% and 60% positive cases, respectively). Thus, antibody 3A1 is a valuable probe for the identification of T lymphoblastic neoplasms since its target antigen is consistently expressed and does not require prior T beta gene rearrangement. Furthermore, expression of 3A1 prior to T beta gene rearrangement suggests that it may be a cell surface protein that participates in the triggering of T cell receptor gene rearrangement and expression. It is concluded that precursor T cell neoplasms display an early T cell development hierarchy that, in sequence, consists of 3A1 expression, T beta gene rearrangements, and surface T3 expression.
前体T（淋巴细胞性）肿瘤反映了T细胞成熟早期阶段的表型。在本研究中，对一系列此类肿瘤进行了分析，以揭示阶段相关细胞表面标志物表达与T细胞受体基因重排之间的发育关联。在大多数（但并非全部，88%）T细胞淋巴细胞性肿瘤病例中发现了T细胞受体β链（Tβ）基因重排。Tβ基因重排在T细胞受体相关分子复合物T3的表面表达之前发生。在所有测试的单克隆抗T细胞抗体中，只有抗体3A1能够与所有病例的肿瘤细胞发生反应，而不论T细胞受体基因重排是否发生。相比之下，通常由成熟T细胞表达的标志物T1和T11在许多病例的肿瘤细胞中缺失（分别为73%和60%的阳性病例）。因此，抗体3A1是鉴定T淋巴细胞性肿瘤的有价值探针，因为其靶抗原持续表达，且不需要预先的Tβ基因重排。此外，Tβ基因重排之前3A1的表达表明它可能是一种参与触发T细胞受体基因重排和表达的细胞表面蛋白。结论是，前体T细胞肿瘤显示出早期T细胞发育层次，依次包括3A1表达、Tβ基因重排和表面T3表达。
