Pittaluga S, Uppenkamp M, Cossman J
Blood. 1987 Apr;69(4):1062-7.
Acquisition of mature T cell function and the T cell antigen receptor repertoire occur in the thymus. In an effort to delineate the cascade of events leading to T cell maturation, we analyzed a series of clonal human precursor T cell neoplasms representing early, middle, and late stages of intrathymic differentiation. Rearrangements of the T cell receptor beta and gamma genes appear concurrently and are preceded by surface expression of the 3A1 (CD7) molecule. Subsequent transcription of the beta gene is coordinated with surface expression of T1 (CD5) and T11 (CD2), transcription of T3 delta mRNA, and the appearance of intracellular T3 (CD3) protein. As late events, T alpha gene transcripts appear and, finally, T3, the multichain complex linked to the T cell receptor, is presented on the cell surface. Findings reported here provide a model of the developmental orchestration of genes encoding antigen recognition in human T cells.
成熟T细胞功能的获得以及T细胞抗原受体库在胸腺中形成。为了描绘导致T细胞成熟的一系列事件,我们分析了一系列代表胸腺内分化早期、中期和晚期的克隆性人类前体T细胞肿瘤。T细胞受体β和γ基因的重排同时出现,且在3A1(CD7)分子的表面表达之前。随后,β基因的转录与T1(CD5)和T11(CD2)的表面表达、T3δmRNA的转录以及细胞内T3(CD3)蛋白的出现相协调。作为后期事件,Tα基因转录本出现,最后,与T细胞受体相连的多链复合物T3呈现在细胞表面。此处报道的研究结果提供了一个人类T细胞中编码抗原识别的基因发育调控模型。