Mencucci Rita, Favuzza Eleonora, Boccalini Carlotta, Lapucci Andrea, Felici Roberta, Resta Francesco, Chiarugi Alberto, Cavone Leonardo
Eye Clinic, Department of Medicine and Translational Surgery, University of Florence, Florence, Italy.
Department of Health Sciences-Section of Pharmacology, University of Florence, Florence, Italy.
Invest Ophthalmol Vis Sci. 2014 Oct 9;55(11):7266-71. doi: 10.1167/iovs.14-15306.
We evaluated the potential protective effects of Coenzyme Q10 (CoQ10) on human corneal cells and rabbit eyes after ultraviolet B (UVB) exposure and a model of wound healing in rabbit eyes after corneal epithelium removal.
Human corneal epithelium cells (HCE) were exposed to a source of UVB radiation (312 nM) in the presence of different CoQ10 concentrations or vehicle. The mitochondrial function and cell survival were evaluated by means of 3-(4,5-dimethylthiazole-2-yl)2,5-diphenyl-tetrazolium (MTT) reduction and lactic dehydrogenase (LDH) release. Furthermore, quantitation of oxygen consumption and mitochondrial membrane potential were conducted. In vivo rabbit models were adopted to evaluate the effect of CoQ10 on UVB-induced conjunctival vessel hyperemia and corneal recovery after ethanol induced corneal lesion.
In UVB-exposed HCE cells, CoQ10 addition led to an increased survival rate and mitochondrial function. Furthermore, oxygen consumption was maintained at control levels and adenosine triphosphate (ATP) decline was completely prevented in the CoQ10-treated cells. Interestingly, in an in vivo model, CoQ10 was able dose-dependently to reduce UVB-induced vessel hyperemia. Finally, in a model of corneal epithelium removal, 12 hours from surgery, animals treated with CoQ10 showed a reduction of damaged area in respect to vehicle controls, which lasted until 48 hours.
We demonstrated that CoQ10 reduces corneal damages after UVB exposure in vivo and in vitro by preserving mitochondrial function. Also, for the first time to our knowledge we showed that the administration of CoQ10 after corneal epithelium removal promotes corneal wound healing.
我们评估了辅酶Q10（CoQ10）对紫外线B（UVB）照射后的人角膜细胞和兔眼以及兔眼角膜上皮去除后伤口愈合模型的潜在保护作用。
在不同CoQ10浓度或赋形剂存在的情况下，将人角膜上皮细胞（HCE）暴露于UVB辐射源（312 nM）。通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑（MTT）还原和乳酸脱氢酶（LDH）释放来评估线粒体功能和细胞存活。此外，还进行了氧消耗和线粒体膜电位的定量分析。采用体内兔模型评估CoQ10对UVB诱导的结膜血管充血以及乙醇诱导角膜损伤后角膜恢复的影响。
在UVB照射的HCE细胞中，添加CoQ10可提高细胞存活率和线粒体功能。此外，CoQ10处理的细胞中氧消耗维持在对照水平，三磷酸腺苷（ATP）下降完全得到预防。有趣的是，在体内模型中，CoQ10能够剂量依赖性地减少UVB诱导的血管充血。最后，在角膜上皮去除模型中，术后12小时，与赋形剂对照组相比，用CoQ10处理的动物受损面积减小，这种情况持续到48小时。
我们证明CoQ10通过保留线粒体功能在体内和体外减少UVB照射后的角膜损伤。此外，据我们所知，我们首次表明角膜上皮去除后给予CoQ10可促进角膜伤口愈合。
