Mirani Zulfiqar Ali, Fatima Aiman, Urooj Shaista, Aziz Mubashir, Khan Mohammad Naseem, Abbas Tanveer
Microbiology Analytical Centre, PCSIR Laboratories Complex Karachi, Pakistan.
Department of Microbiology, University of Karachi, Pakistan.
Iran J Basic Med Sci. 2018 Jul;21(7):760-769. doi: 10.22038/IJBMS.2018.28525.6917.
This study was designed to determine the relationship of and isolates in multispecies biofilms and their individual phenotypic characters in biofilm consortia.
The subject isolates were recovered from different food samples and identified on the basis of growth on differential and selective media. Tube methods, Congo-red agar method, and scanning electron microscopy (SEM) were used to study biofilms phenotypes. The hydrophobicity of the strains was evaluated by the adhesion to apolar solvent.
The results showed that dominated the pre-biofilm stage. It has been observed that adopted biofilm life much before and . However, after adopting biofilm lifestyle, slowly and gradually, dominated the consortia and dispersed other stakeholders. The subject isolates of produce cis-2-decanoic acid to disperse or inhibit and biofilms. Gas-chromatography and mass spectrometry results showed that cis-2-decanoic was higher in the co-culture condition and increased at late log-phase or at stationary phase. Although majority of were unable to compete with , however, a minor population competed, survived, and persisted in biofilm consortia as small colony variants. The survivors showed higher expression of sigB and sarA genes. showed comparatively higher hydrophobic surface properties.
Comparative analysis showed that cell surface hydrophobicity, growth rate, and small colony variants (SCVs) are correlated in biofilm consortia of the and
本研究旨在确定多物种生物膜中[具体菌种1]和[具体菌种2]分离株之间的关系及其在生物膜群落中的个体表型特征。
从不同食品样本中分离出受试菌株，并根据其在鉴别和选择性培养基上的生长情况进行鉴定。采用试管法、刚果红琼脂法和扫描电子显微镜（SEM）研究生物膜表型。通过菌株对非极性溶剂的粘附性评估其疏水性。
结果表明，[具体菌种1]在生物膜形成前阶段占主导地位。据观察，[具体菌种1]比[具体菌种2]和[具体菌种3]更早采用生物膜生活方式。然而，在采用生物膜生活方式后，[具体菌种2]逐渐在群落中占主导地位并驱散了其他菌种。受试的[具体菌种1]分离株产生顺式-2-癸酸以驱散或抑制[具体菌种2]和[具体菌种3]的生物膜。气相色谱和质谱结果表明，顺式-2-癸酸在共培养条件下含量更高，在对数后期或稳定期增加。虽然大多数[具体菌种3]无法与[具体菌种2]竞争，但一小部分[具体菌种3]作为小菌落变体在生物膜群落中竞争、存活并持续存在。存活者显示出sigB和sarA基因的高表达。[具体菌种2]表现出相对较高的疏水表面特性。
比较分析表明，在[具体菌种1]和[具体菌种2]的生物膜群落中，细胞表面疏水性、生长速率和小菌落变体（SCV）之间存在相关性。
