Chen Ling-Ling, Chen Feng, Zhao Hua-Zhu, Feng Zhao-Zhong, Zhang Hao, Huang Xing
1College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
2School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, PR China.
Int J Syst Evol Microbiol. 2018 Oct;68(10):3296-3300. doi: 10.1099/ijsem.0.002986. Epub 2018 Aug 24.
A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain, designated D6, was isolated from farmland soil in Xuancheng, Anhui Province, China. Growth occurred at 4-37 °C (optimum, 30 °C), at pH 6.5-8.5 (optimum, 7.0) and with 0-7 % (w/v) NaCl (optimum, 0.5 % NaCl). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D6 was most closely related to Aestuariimicrobium kwangyangense DSM 21549 (98.47 %), followed by Tessaracoccus rhinocerotis YIM 101269 (94.46 %). Strain D6 had a cell-wall peptidoglycan based on ll-diaminopimelic acid. MK-9(H4) was the predominant menaquinone. The major fatty acids of strain D6 were anteiso-C15 : 0, iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B). The major polar lipids were a lipid, glycolipid and phospholipid. The DNA G+C content was 69.2 mol% and strain D6 showed low DNA-DNA relatedness to A. kwangyangense DSM 21549 (36.45±0.42 %). Based on these genotypic and phenotypic data, strain D6 represents a novel species in the genus Aestuariimicrobium, for which the name Aestuariimicrobium soli sp. nov. is proposed. The type strain is D6 (=KCTC 39995=DSM 105824). An emended description of the genus Aestuariimicrobium is presented.
从中国安徽省宣城市的农田土壤中分离出一株革兰氏反应阳性、过氧化氢酶阳性、无芽孢形成且呈短杆状或椭圆形的细菌菌株，命名为D6。该菌株在4-37°C（最适温度为30°C）、pH值6.5-8.5（最适pH值为7.0）以及0-7%（w/v）NaCl（最适NaCl浓度为0.5%）的条件下生长。基于16S rRNA基因序列的系统发育分析表明，菌株D6与光阳河口微菌DSM 21549（相似度为98.47%）关系最为密切，其次是犀牛球孢菌YIM 101269（相似度为94.46%）。菌株D6的细胞壁肽聚糖基于L-二氨基庚二酸。MK-9（H4）是主要的甲基萘醌。菌株D6的主要脂肪酸为anteiso-C15:0、iso-C15:0和特征性脂肪酸总和4（iso-C17:1 I和/或anteiso-C17:1 B）。主要极性脂类为一种脂类、糖脂和磷脂。DNA的G+C含量为69.2 mol%，且菌株D6与光阳河口微菌DSM 21549的DNA-DNA相关性较低（36.45±0.42%）。基于这些基因型和表型数据，菌株D6代表了河口微菌属中的一个新物种，为此提出名为土壤河口微菌新种（Aestuariimicrobium soli sp. nov.）。模式菌株为D6（=KCTC 39995=DSM 105824）。本文还给出了河口微菌属的修订描述。
