Key Laboratory for Conservation and Utilization of Bio-Resource, and Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.
Arch Microbiol. 2021 Jul;203(5):2653-2658. doi: 10.1007/s00203-021-02261-2. Epub 2021 Mar 12.
A novel Gram-stain positive, oval-shaped, and non-flagellated bacterium, designated YIM S02566, was isolated from alpine soil in Shadui Towns, Ganzi County, Ganzi Tibetan Autonomous Prefecture, Sichuan Province, PR China. Growth occurred at 23-35 °C (optimum, 30 °C) in the presence of 0.5-4% (w/v) NaCl (optimum, 1%) and at pH 7.0-8.0 (optimum, pH 7.0). The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain YIM S02566 was most closely related to the genus Aestuariimicrobium, with Aestuariimicrobium kwangyangense R27 and Aestuariimicrobium soli D6 as its closest relative (sequence similarities were 96.3% and 95.4%, respectively). YIM S02566 contained LL-diaminopimelic acid in the cell wall. MK-9(H4) was the predominant menaquinone. The major fatty acid patterns were anteiso-C (60.0%). The major polar lipid was DPG. The genome size of strain YIM S02566 was 3.1 Mb, comprising 3078 predicted genes with a DNA G + C content of 69.0 mol%. Based on these genotypic, chemotaxonomic and phenotypic evidences, strain YIM S02566 was identified as a novel species in the genus Aestuariimicrobium, for which the name Aestuariimicrobium ganziense sp. nov. is proposed. The type strain is YIM S02566 (= CGMCC 1.18751 T = KCTC 49,477 T).
一株新的革兰氏阳性、椭圆形、无鞭毛的细菌，被命名为 YIM S02566，从中国四川省甘孜藏族自治州甘孜县沙堆镇的高山土壤中分离得到。该菌在 23-35°C（最佳温度为 30°C）、0.5-4%（w/v）NaCl（最佳浓度为 1%）和 pH7.0-8.0（最佳 pH 值为 7.0）的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 YIM S02566 与 Aestuariimicrobium 属最为密切相关，与 Aestuariimicrobium kwangyangense R27 和 Aestuariimicrobium soli D6 的亲缘关系最近（序列相似度分别为 96.3%和 95.4%）。YIM S02566 的细胞壁中含有 LL-二氨基庚二酸。MK-9(H4) 是主要的menaquinone。主要的脂肪酸模式为 anteiso-C（60.0%）。主要的极性脂是 DPG。菌株 YIM S02566 的基因组大小为 3.1 Mb，包含 3078 个预测基因，其 DNA G+C 含量为 69.0 mol%。基于这些基因型、化学分类学和表型证据，菌株 YIM S02566 被鉴定为 Aestuariimicrobium 属的一个新种，命名为 Aestuariimicrobium ganziense sp. nov.。模式菌株为 YIM S02566（=CGMCC 1.18751T=KCTC 49477T）。
