Murru Francesca, Fliegerova Katerina, Mura Erica, Mrázek Jakub, Kopečný Jan, Moniello Giuseppe
Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy.
Institute of Animal Physiology and Genetics, CAS, v.v.i., Vídeňská 1083, Prague 14220, Czech Republic.
Anaerobe. 2018 Dec;54:104-110. doi: 10.1016/j.anaerobe.2018.08.009. Epub 2018 Aug 22.
The diversity of the methanogenic archaea associated with the six segments of the horse and donkey hindgut (caecum, right ventral colon, left ventral colon, left dorsal colon, right dorsal colon, and rectum) was analyzed using 16S rDNA gene clone library. A total of 641 positive clones, 321 from the horse and 320 from the donkey hindgut, were examined by the RFLP, revealing 9 different ribotypes, 8 in the horse and 5 in the donkey hindgut. In both the animals Methanobacteriales (Methanobrevibacter-like sequences) and Methanomicrobiales (Methanocorpusculum-like sequences) were detected as the dominant orders followed by the uncultured Methanomassiliicoccales. The composition of the equine archaeal community was found to be dependent on the gut region. In both the two animals no Methanobrevibacter-like clones were detected in the caeca, which were instead inhabited by the Methanocorpusculum-like archeons. The Methanosarcinales were found only in distal regions of the horse hindgut.
利用16S rDNA基因克隆文库分析了与马和驴后肠六个节段（盲肠、右腹结肠、左腹结肠、左背结肠、右背结肠和直肠）相关的产甲烷古菌的多样性。通过RFLP分析了总共641个阳性克隆，其中321个来自马后肠，320个来自驴后肠，揭示了9种不同的核糖型，马后肠中有8种，驴后肠中有5种。在这两种动物中，均检测到甲烷杆菌目（类短柄甲烷杆菌序列）和甲烷微菌目（类球状甲烷菌序列）为优势菌目，其次是未培养的甲烷球形菌目。发现马古菌群落的组成取决于肠道区域。在这两种动物的盲肠中均未检测到类短柄甲烷杆菌克隆，取而代之的是类球状甲烷菌古菌。甲烷八叠球菌目仅在马后肠的远端区域发现。
