de Oliveira Rodrigo Cardoso, de Oliveira Fabio Henrique Gelonese, Cestari Tânia Mary, Taga Rumio, Granjeiro José Mauro
Institute of Biology, UNICAMP, Bauru Dental School, University of São Paulo, Brazil.
Clin Oral Implants Res. 2008 Aug;19(8):749-54. doi: 10.1111/j.1600-0501.2008.01521.x.
To evaluate the repair of critical-size bone defects in rats treated with demineralized bovine bone (DBB) compared with autogenous bone (AB).
A bone defect of 8 mm in diameter was created in the calvaria of 50 Rattus norvegicus, treated either with DBB or AB. Sub-groups of five rats of each group were killed at 7, 14, 21, 30 and 90 days post-operatively, and the skulls were removed and processed histologically. Histological sections were stained with hematoxylin and eosin.
Histological analysis showed complete closure of the defects with new bone at 90 days in group AB, and substitution of the biomaterial by fibrotic connective tissue in the DBB group at 21 days. Morphometric analysis showed that DBB was rapidly absorbed at 14 days, with its volume density decreasing from 47%+/-0.8% at 7 days to 1.2%+/-0.41% at 14 days. Subsequently, volume densities of the connective tissue and neoformed bone increased from 51.1%+/-11.17% to 86.8%+/-7.92% and from 1.9%+/-1.13% to 12%+/-8.02%, respectively, for the same time interval. The volume density of AB particles did not change throughout the experimental periods, but the amount of new bone increased markedly between 7 and 90 days, from 4.5%+/-1.57% to 53.5%+/-6.42% (P<0.05).
DBB did not provide complete repair of the defects, with significantly less new bone formation than in the AB group.
评估脱矿牛骨（DBB）与自体骨（AB）治疗大鼠临界尺寸骨缺损的修复情况。
在50只挪威大鼠的颅骨上制造直径8mm的骨缺损，分别用DBB或AB治疗。每组五只大鼠的亚组在术后7、14、21、30和90天处死，取出颅骨并进行组织学处理。组织学切片用苏木精和伊红染色。
组织学分析显示，AB组在90天时缺损完全被新骨封闭，而DBB组在21天时生物材料被纤维结缔组织替代。形态计量分析显示，DBB在14天时迅速吸收，其体积密度从7天时的47%±0.8%降至14天时的1.2%±0.41%。随后，在相同时间间隔内，结缔组织和新形成骨的体积密度分别从51.1%±11.17%增加到86.8%±7.92%，从1.9%±1.13%增加到12%±8.02%。AB颗粒的体积密度在整个实验期间没有变化，但新骨量在7至90天之间显著增加，从4.5%±1.57%增加到53.5%±6.42%（P<0.05）。
DBB未能完全修复缺损，新骨形成明显少于AB组。
