Troszok Agnieszka, Kolek Ludmiła, Szczygieł Joanna, Wawrzeczko Joanna, Borzym Ewa, Reichert Michał, Kamińska Teresa, Ostrowski Tomasz, Jurecka Patrycja, Adamek Mikołaj, Rakus Krzysztof, Irnazarow Ilgiz
Institute of Ichthyobiology and Aquaculture, Polish Academy of Sciences, Chybie, Poland.
Department of Fish Diseases, National Veterinary Research Institute in Puławy, Puławy, Poland.
J Fish Dis. 2018 Nov;41(11):1709-1718. doi: 10.1111/jfd.12880. Epub 2018 Aug 24.
Cyprinid herpesvirus 3 (CyHV-3), also known as koi herpesvirus (KHV), is an aetiological agent of a virulent and lethal disease in common and koi carp. In this study, we examined in vitro the anti-CyHV-3 activity of acyclovir (ACV), nucleoside analogue commonly used against human herpesviruses, as well as acyclovir monophospate (ACV-MP). The cytotoxicity of the ACV and the ACV-MP for two common carp cell lines, CCB (Common carp brain) and KF1 (Koi carp fin 1), was determined by means of MTT and crystal violet assays. In subsequent studies, the concentration of 66.67 μM was applied. The ACV and the ACV-MP (66.67 μM) inhibited a cytopathic effect (CPE) induced by the CyHV-3 virus in the CCB (ACV by 66%, ACV-MP by 58%) and the KF1 (ACV by 25%, ACV-MP by 37%). The viral load measured by the means of TaqMan qPCR was reduced in a range of 67%-93% depending on the analogue, the cell line and the time of incubation. The expression of viral genes (ORF149, ORF3, ORF134 and ORF78) in CCB cells infected with the CyHV-3 was strongly downregulated within the range of 78%-91%. In summary, both the ACV and the ACV-MP can inhibit CyHV-3 replication in vitro.
鲤疱疹病毒3型（CyHV-3），也被称为锦鲤疱疹病毒（KHV），是一种导致普通鲤鱼和锦鲤患上烈性致命疾病的病原体。在本研究中，我们在体外检测了阿昔洛韦（ACV）（一种常用于对抗人类疱疹病毒的核苷类似物）以及单磷酸阿昔洛韦（ACV-MP）对CyHV-3的抗病毒活性。通过MTT和结晶紫测定法确定了ACV和ACV-MP对两种鲤鱼细胞系CCB（鲤鱼脑）和KF1（锦鲤鳍1）的细胞毒性。在后续研究中，采用了66.67μM的浓度。ACV和ACV-MP（66.67μM）抑制了CyHV-3病毒在CCB（ACV抑制66%，ACV-MP抑制58%）和KF1（ACV抑制25%，ACV-MP抑制37%）中诱导的细胞病变效应（CPE）。根据类似物、细胞系和孵育时间的不同，通过TaqMan qPCR测量的病毒载量降低了67%-93%。在感染CyHV-3的CCB细胞中，病毒基因（ORF149、ORF3、ORF134和ORF78）的表达在78%-91%的范围内被强烈下调。总之，ACV和ACV-MP均可在体外抑制CyHV-3的复制。
