Faculty of Animal Science and Food Engineering, University of Sao Paulo, Avenue Duque de Caxias Norte, Jardim Elite, Pirassununga, Sao Paulo, 225, Brazil.
Faculty of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil.
Braz J Microbiol. 2022 Dec;53(4):1807-1815. doi: 10.1007/s42770-022-00797-z. Epub 2022 Jul 22.
Cyprinid herpesvirus 3 has a worldwide distribution and presents high mortality rates in species of Cyprinus carpio, causing serious economic loss to the global aquaculture industry. The description of this infection in other ornamental fish species is still limited. For this purpose, 100 ornamental fish from 24 different species were tested by PCR for Cyprinid hespesvirus 3 and the positive samples represented 6% of the tested samples. Phylogenetic reconstruction, based on the Thymidine Kinase gene, revealed the existence of two distinct clades. One clade grouped a Brazilian sample with European and Asian genotypes of CyHV-3 and a second clade, containing only Brazilian sequences described in this study. All of the Brazilian sequences showed identity values greater than 97.7% when compared to each other. This is the first report of the occurrence of Cyprinid herpesvirus 3 in ornamental fish species in Brazil. These results in association with further studies of viral isolation and characterization can help in establishing effective surveillance and disease control program.
锦鲤疱疹病毒 3 在世界各地分布广泛，在鲤鱼属的物种中引起高死亡率，给全球水产养殖业造成严重的经济损失。其他观赏鱼物种中这种感染的描述仍然有限。为此，通过 PCR 对 24 个不同物种的 100 条观赏鱼进行了锦鲤疱疹病毒 3 的检测，阳性样本占检测样本的 6%。基于胸苷激酶基因的系统发育重建显示存在两个不同的分支。一个分支将一个巴西样本与欧洲和亚洲的锦鲤疱疹病毒 3 基因型聚集在一起，第二个分支仅包含本研究中描述的巴西序列。与其他序列相比，所有巴西序列的同一性值均大于 97.7%。这是巴西首次报告在观赏鱼物种中发生锦鲤疱疹病毒 3。这些结果与进一步的病毒分离和特征研究相结合，可以帮助建立有效的监测和疾病控制计划。
