Park Jihyun, Kim Jiwoo, Hwang Sungwon, Chung Ki Young, Choi Inho, Choi Chang Bon, Kim Jihoe
Department of Medical Biotechnology, Yeungnam University, Gyeongsan 38541, Korea.
Hanwoo Experiment Station, National Institute of Animal Science, RDA, Pyongchang 25340, Korea.
Asian-Australas J Anim Sci. 2019 Mar;32(3):437-441. doi: 10.5713/ajas.18.0138. Epub 2018 Aug 27.
Paraoxonase 1 (PON1), a calcium-dependent serum enzyme, has been shown to be involved in lipid metabolism. In this study, we examined the putative correlation of the serum PON1 level of Hanwoo, Korean native cattle, with gender and meat quality grade.
PON1 levels were estimated by determining the arylesterase and paraoxonase activities (AE and PO, respectively) in serum samples from Hanwoo individuals (n = 56). Serum PON1 levels were analyzed in different gender groups (female [n = 21], castrated male [n = 17], and male [n = 18]), and meat quality grades (≥1 [n = 23], 2 [n = 21], and 3 [n = 12]).
Serum PON1 levels were similar in female (AE = 120±55 U/mL, PO = 84±43 mU/mL) and castrated male (123±44 U/mL, PO = 89±30 mU/mL), while male showed a significantly lower level (AE = 65±43 U/mL, PO = 44±34 mU/mL). Furthermore, analysis of serum PON1 levels in three different grades of meat quality showed similar levels in the grades ≥1 (AE = 118±49 U/mL, PO = 84±37 mU/mL) and 2 (AE = 116±54 U/mL, PO = 82±43 mU/mL), while the level was significantly lower in the grade 3 (AE = 58±35 U/mL, PO = 39±27 mU/mL) of lower meat quality.
We discovered the gender-dependent differences in serum PON1 levels of Hanwoo and a positive association of the serum PON1 level with meat quality. Results in this study suggest that PON1 would be a useful serum marker for preliminary screening of Hanwoo individuals with high-quality meat and applicable for genetic improvement.
对氧磷酶1（PON1）是一种钙依赖性血清酶，已被证明参与脂质代谢。在本研究中，我们检测了韩牛（韩国本土牛）血清PON1水平与性别和肉质等级之间的潜在相关性。
通过测定韩牛个体（n = 56）血清样本中的芳基酯酶和对氧磷酶活性（分别为AE和PO）来估计PON1水平。分析了不同性别组（雌性[n = 21]、去势雄性[n = 17]和雄性[n = 18]）以及不同肉质等级（≥1级[n = 23]、2级[n = 21]和3级[n = 12]）的血清PON1水平。
雌性（AE = 120±55 U/mL，PO = 84±43 mU/mL）和去势雄性（AE = 123±44 U/mL，PO = 89±30 mU/mL）的血清PON1水平相似，而雄性的水平显著较低（AE = 65±43 U/mL，PO = 44±34 mU/mL）。此外，对三种不同肉质等级的血清PON1水平分析显示，≥1级（AE = 118±49 U/mL，PO = 84±37 mU/mL）和2级（AE = 116±54 U/mL，PO = 82±43 mU/mL）的水平相似，而肉质较低的3级（AE = 58±35 U/mL，PO = 39±27 mU/mL）的水平显著较低。
我们发现了韩牛血清PON1水平的性别依赖性差异以及血清PON1水平与肉质的正相关。本研究结果表明，PON1将是用于初步筛选具有高品质肉的韩牛个体的有用血清标志物，并可用于遗传改良。
