Abdallah Emad, El-Shishtawy Samya, Sherif Nevine, Ali Ahmed, El-Bendary Omnia
Department of Nephrology, Theodor Bilharz Research Institute, El-Nile St. Waraq El-Hadar, P.O. Box 30, Imbaba, Giza, 12411, Egypt.
Department of Intensive Care Unit, Theodor Bilharz Research Institute, Cairo, Egypt.
Int Urol Nephrol. 2017 Feb;49(2):329-335. doi: 10.1007/s11255-016-1465-y. Epub 2016 Nov 23.
Paraoxonase-1 (PON-1) is a high-density lipoprotein-associated (HDL) enzyme, which has been shown to reduce susceptibility to low-density lipoprotein (LDL) peroxidation. Epicardial adipose tissue (EAT) is a marker of atherosclerosis. The aim of this study was to determine the relationship between PON-1 activity and EAT in hemodialysis (HD) patients.
This is a cross-sectional study conducted on 72 (43 males) HD patients with end-stage renal disease. Serum levels for lipid profiles, C-reactive protein, calcium, phosphate, and parathyroid hormone were measured. PON-1 activity was also measured and compared to the rate of enzymatic hydrolysis of paraoxon to p-nitrophenol. Echocardiography was used to measure EAT thickness (EATT). The correlation between PON-1 and EATT was assessed, while independent predictors of EATT in HD patients were similarly assessed using multivariate regression analysis.
There was a significant low mean value of PON-1 activity in HD patients compared with the control group (82.1 ± 31.6 vs. 164.3 ± 61.5 U/l, p = 0.0001) and significant high mean value of EATT in HD patients, compared with controls (6.2 ± 1.7 vs. 3.9 ± 1.1 mm, p = 0.0001). In addition, there was a significant negative correlation between PON-1 activity and EATT (r = -0.484, p = 0.0001) and a significant positive correlation between PON-1 activity and HDL-C (r = 0.417, p = 0.0003). PON-1, total cholesterol, triglycerides, LDL, HDL, age, and body mass index were found to be independent predictors of EATT.
Our study demonstrated that PON-1 activity was significantly lower in HD patients compared with healthy controls and that PON-1 activity was inversely correlated with EATT in this population.
对氧磷酶-1（PON-1）是一种与高密度脂蛋白（HDL）相关的酶，已被证明可降低低密度脂蛋白（LDL）过氧化的易感性。心外膜脂肪组织（EAT）是动脉粥样硬化的一个标志物。本研究的目的是确定血液透析（HD）患者中PON-1活性与EAT之间的关系。
这是一项对72例（43例男性）终末期肾病HD患者进行的横断面研究。测量了血脂谱、C反应蛋白、钙、磷和甲状旁腺激素的血清水平。还测量了PON-1活性，并将其与对氧磷酶水解为对硝基苯酚的酶促水解速率进行比较。使用超声心动图测量EAT厚度（EATT）。评估了PON-1与EATT之间的相关性，同时使用多变量回归分析类似地评估了HD患者中EATT的独立预测因素。
与对照组相比，HD患者的PON-1活性平均显著降低（82.1±31.6 vs. 164.3±61.5 U/l，p = 0.0001），与对照组相比，HD患者的EATT平均显著升高（6.2±1.7 vs. 3.9±1.1 mm，p = 0.0001）。此外，PON-1活性与EATT之间存在显著负相关（r = -0.484，p = 0.0001），PON-1活性与HDL-C之间存在显著正相关（r = 0.417，p = 0.0003）。发现PON-1、总胆固醇、甘油三酯、LDL、HDL、年龄和体重指数是EATT的独立预测因素。
我们的研究表明，与健康对照组相比，HD患者的PON-1活性显著降低，且该人群中PON-1活性与EATT呈负相关。
