Piletz J E, Andorn A C, Halaris A
Psychopharmacology (Berl). 1986;89(3):370-7. doi: 10.1007/BF00174377.
3H-Clonidine and 3H-yohimbine were observed to bind to glass fiber filters. The binding was displaced by co-filtration with the corresponding non-radioactive ligand. Phentolamine and (--)-norepinephrine were ineffective in displacing either 3H-clonidine or 3H-yohimbine bound to filters. Failure to correct for filter binding resulted in an over-estimation of specific binding to platelet membranes. Certain published methodologies may have consequently misidentified up to 20% of the specific binding to platelets, that was actually due to displaced filter binding. Experimental conditions are described which eliminate filter binding. These results are significant for the interpretation of data from studies of platelet binding in depressed patients.
观察到3H-可乐定和3H-育亨宾与玻璃纤维滤膜结合。通过与相应的非放射性配体共过滤可使这种结合发生位移。酚妥拉明和（-）-去甲肾上腺素在置换与滤膜结合的3H-可乐定或3H-育亨宾方面无效。未对滤膜结合进行校正导致对血小板膜特异性结合的高估。某些已发表的方法可能因此错误地将高达20%的血小板特异性结合鉴定为实际上是由于滤膜结合位移所致。文中描述了消除滤膜结合的实验条件。这些结果对于解释抑郁症患者血小板结合研究的数据具有重要意义。
