Ge Hua, Yan Yan, Wu Di, Huang Yongsheng, Tian Fei
Department of Gastrointestinal Surgery, The First People's Hospital of Zunyi, Zunyi, Guizhou, People's Republic of China,
Quality Control Department, The First People's Hospital of Zunyi, Zunyi, Guizhou, People's Republic of China.
Onco Targets Ther. 2018 Aug 14;11:4845-4855. doi: 10.2147/OTT.S173225. eCollection 2018.
The clinical significance of LINC00996 in colorectal cancer (CRC) has not been verified. In the current study, the authors aimed to explore the expression of LINC00996 and its clinical significance in CRC based on the data mining of Gene Expression Omnibus (GEO) and the Cancer Genome Atlas (TCGA) datasets, as well as to elucidate the functions of its potential target genes.
GEO and TCGA microarray datasets were used to evaluate the LINC00996 expression and its clinical significance in CRC. LINC00996 related genes were identified by Multi Experiment Matrix, RNA-Binding Protein DataBase, and The Atlas of Noncoding RNAs in Cancer. Subsequently, they were sent to gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analysis.
LINC00996 is significantly decreased in CRC tissues compared with non-tumor tissues. Low level of LINC00996 is associated with remote metastasis and poor overall survival. However, LINC00996 has a minimal effect on gender, lymphatic invasion, tumor size, lymph node metastasis, and pathological stage. One hundred and forty-two LINC00996 related genes were identified; the results of functional analysis indicated that LINC00996 might repress tumorigenesis and metastasis via modulating the JAK-STAT, NF-κB, HIF-1, TLR, and PI3K-AKT signaling pathways.
Our study demonstrates that decreased LINC00996 expression may be involved in colorectal carcinogenesis and metastasis, and the depletion of LINC00996 is associated with a poor outcome in CRC patients. Moreover, the JAK-STAT, NF-κB, HIF-1, TLR, and PI3K-AKT pathways may be the key pathways regulated by LINC00996 in CRC.
LINC00996在结直肠癌（CRC）中的临床意义尚未得到证实。在本研究中，作者旨在基于基因表达综合数据库（GEO）和癌症基因组图谱（TCGA）数据集的数据挖掘，探讨LINC00996在CRC中的表达及其临床意义，并阐明其潜在靶基因的功能。
使用GEO和TCGA微阵列数据集评估LINC00996在CRC中的表达及其临床意义。通过多实验矩阵、RNA结合蛋白数据库和癌症非编码RNA图谱鉴定LINC00996相关基因。随后，将它们送去进行基因本体富集和京都基因与基因组百科全书通路分析。
与非肿瘤组织相比，LINC00996在CRC组织中显著降低。LINC00996水平低与远处转移和总体生存率差相关。然而，LINC00996对性别、淋巴浸润、肿瘤大小、淋巴结转移和病理分期影响极小。鉴定出142个LINC00996相关基因；功能分析结果表明，LINC00996可能通过调节JAK-STAT、NF-κB、HIF-1、TLR和PI3K-AKT信号通路来抑制肿瘤发生和转移。
我们的研究表明，LINC00996表达降低可能参与了结直肠癌的发生和转移，LINC00996的缺失与CRC患者的不良预后相关。此外，JAK-STAT、NF-κB、HIF-1、TLR和PI3K-AKT通路可能是LINC00996在CRC中调节的关键通路。
