Morishima Y, Ohya K, Ueda R, Fukuda T
Vox Sang. 1986;50(4):212-5. doi: 10.1111/j.1423-0410.1986.tb04883.x.
Adult T cell leukemia associated antibody (ATLA-Ab) positive persons were screened by indirect immunofluorescence (IF) testing. Their lymphocytes were collected from concentrated red blood cells (CRC), and cultured in vitro with and without phytohemagglutinin (PHA) for 10 days. The expression of ATL virus (ATLV) positive lymphocytes during the in vitro culture was then analyzed by IF assay using mouse monoclonal antibody ATL-19 reactive to p19 core protein of ATLV. 97% of ATLA-Ab positive CRC (36 cases) demonstrated ATLV positive lymphocytes after being cultured for more than 10 days with PHA, whereas, none of ATLA-Ab negative CRC (22 cases) demonstrated ATLV positive lymphocytes. All of the 10 ATLA-Ab positive CRC that were stored for 2, 4, and 7 days contained lymphocytes which expressed ATLV after in vitro culture, while 7 of 10 CRC stored for 14 days and only 1 of 10 CRCs stored for 20 days, expressed ATLV positive lymphocytes. This data indicates that almost all of the ATLA-Ab positive blood contained ATLV positive lymphocytes, and that the in vitro appearance of these ATLV positive lymphocytes was reduced by storing the CRC for more than 14 days.
采用间接免疫荧光（IF）检测对成人T细胞白血病相关抗体（ATLA - Ab）阳性者进行筛查。从浓缩红细胞（CRC）中采集其淋巴细胞，分别在有和无植物血凝素（PHA）的条件下进行体外培养10天。然后使用对ATLV的p19核心蛋白有反应的小鼠单克隆抗体ATL - 19，通过IF检测分析体外培养期间ATL病毒（ATLV）阳性淋巴细胞的表达情况。97%的ATLA - Ab阳性CRC（36例）在与PHA一起培养超过10天后显示出ATLV阳性淋巴细胞，而ATLA - Ab阴性CRC（22例）均未显示出ATLV阳性淋巴细胞。10例保存2天、4天和7天的ATLA - Ab阳性CRC中所有样本的淋巴细胞在体外培养后均表达ATLV，而在保存14天的10例CRC中有7例以及保存20天的10例CRC中仅有1例表达ATLV阳性淋巴细胞。该数据表明，几乎所有ATLA - Ab阳性血液都含有ATLV阳性淋巴细胞，并且通过将CRC保存超过14天，这些ATLV阳性淋巴细胞的体外出现率会降低。
