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长期盐胁迫下草莓抗氧化酶活性及一些形态生理特征的变化

Change in antioxidant enzymes activity and some morpho-physiological characteristics of strawberry under long-term salt stress.

作者信息

Ghaderi Nasser, Hatami Mohamad Reza, Mozafari Aliakbar, Siosehmardeh Adel

机构信息

1Department of Horticultural Sciences, Agricultural Faculty, University of Kurdistan, P. O. Box 416, Sanandej, 66177-15175 Iran.

3Research Center for Strawberry Breeding and Improvement, University of Kurdistan, P. O. Box 416, Sanandaj, 66177-15175 Iran.

出版信息

Physiol Mol Biol Plants. 2018 Sep;24(5):833-843. doi: 10.1007/s12298-018-0535-2. Epub 2018 May 26.

Abstract

The effects of long term salinity on some morpho-physiological characteristics were studied in strawberry Kurdistan and Queen elisa cultivars. Vegetative and biochemical traits were measured in strawberry cultivars subjected to three levels of salinity including 0, 40 and 80 mM at 20, 40 and 60th days after NaCl addition. Results showed that in both cultivars the dry weight of plant organs decreased in response to NaCl, except of crown weight in cv. Kurdistan. Root to shoot ratio increased due to a greater reduction in above ground biomass under salinity. Strawberry cultivars tended to decrease their stomatal conductance, RWC, proline, soluble carbohydrates and proteins during the different evaluation periods. Compared to the 20th day, peroxidase activity decreased at 80 mM during 40 and 60 days in cv. Queen elisa. On the contrary, ascorbate peroxidase activity elevated until the 40th day and decreased afterwards, in addition application of 40 and 80 mM NaCl increased the ascorbate peroxidase activity of both studied cultivars. Catalase activity increased from 20th until 60th days in cv. Queen elisa, while showed increase in cv. Kurdistan until day 40 and then decreased again at day 60. Application of 40 and 80 mM NaCl resulted in an increase in peroxidase, ascorbate peroxidase and catalase activities of both cultivars. The Queen elisa cv. showed lower tolerance index (45.88%) compared with cv. Kurdistan (67.97%). Finally, higher salinity resistance of cv. Kurdistan is probably associated with its ability to maintain higher RWC and higher activity of antioxidant enzymes.

摘要

研究了长期盐度对库尔德斯坦草莓和伊丽莎女王草莓品种某些形态生理特征的影响。在添加氯化钠后的第20、40和60天,对处于0、40和80 mM三个盐度水平下的草莓品种的营养和生化特性进行了测定。结果表明,在两个品种中,除库尔德斯坦品种的冠重外,植物器官的干重均因氯化钠处理而降低。由于盐度下地上生物量的更大减少,根冠比增加。在不同的评估期内,草莓品种的气孔导度、相对含水量、脯氨酸、可溶性碳水化合物和蛋白质含量均呈下降趋势。与第20天相比,伊丽莎女王品种在第40和60天80 mM盐度下过氧化物酶活性降低。相反,抗坏血酸过氧化物酶活性在第40天之前升高,之后降低,此外,40和80 mM氯化钠处理增加了两个研究品种的抗坏血酸过氧化物酶活性。伊丽莎女王品种的过氧化氢酶活性从第20天到第60天增加,而库尔德斯坦品种在第40天之前增加,然后在第60天再次下降。40和80 mM氯化钠处理导致两个品种的过氧化物酶、抗坏血酸过氧化物酶和过氧化氢酶活性增加。伊丽莎女王品种的耐盐指数(45.88%)低于库尔德斯坦品种(67.97%)。最后,库尔德斯坦品种较高的耐盐性可能与其维持较高相对含水量和较高抗氧化酶活性的能力有关。

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