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人类肺腺癌中长非编码 RNA 的基因芯片表达谱。

Microarray expression profile of long non-coding RNAs in human lung adenocarcinoma.

机构信息

Department of Pathology, Weifang Medical University, Weifang, China.

Medicine Research Center, Weifang Medical University, Weifang, China.

出版信息

Thorac Cancer. 2018 Oct;9(10):1312-1322. doi: 10.1111/1759-7714.12845. Epub 2018 Aug 27.

DOI:10.1111/1759-7714.12845
PMID:30151992
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6166069/
Abstract

BACKGROUND

Long non-coding RNAs (lncRNAs) participate in many biological dynamics and play significant roles in gene regulation. LncRNA expression is altered in many cancers; however, the expressions and functions of lncRNA genes in lung adenocarcinoma (LAD) remain unknown.

METHODS

LncRNA and messenger RNA (mRNA) expression in LAD without lymphatic metastasis versus paired adjacent non-tumor (ANT) lung tissues and LAD with versus without lymphatic metastasis were analyzed using Human LncRNA Arraystar V3.0. The expression levels of four downregulated and four upregulated lncRNAs were verified using quantitative real-time PCR in cells and tissue specimens.

RESULTS

In this study, 949 lncRNAs and 681 mRNAs had differential expression in LAD without lymphatic metastasis compared to ANT lung tissues, while 2740 lncRNAs and 1714 mRNAs were differentially expressed in LAD with lymphatic metastasis compared to LAD without lymphatic metastasis. The expression patterns of selected lncRNAs (LINC00113, AC005009.1, ARHGAP22-IT1, AC009411.1, SRGAP3-AS2, EGFEM1P, FAM66E, and HLA-F-AS1) were consistent with microarray data. Differentially expressed mRNA genes were enriched in crucial Gene Ontology terms and pathways.

CONCLUSION

Our results revealed differentially expressed lncRNAs in LAD, suggesting lncRNAs may be potential indicators for LAD diagnosis and therapy.

摘要

背景

长非编码 RNA（lncRNA）参与许多生物学动态过程，并在基因调控中发挥重要作用。许多癌症中 lncRNA 的表达发生改变，但肺腺癌（LAD）中 lncRNA 基因的表达和功能仍不清楚。

方法

使用 Human LncRNA Arraystar V3.0 分析无淋巴结转移的 LAD 与配对的相邻非肿瘤（ANT）肺组织以及有和无淋巴结转移的 LAD 之间的 lncRNA 和信使 RNA（mRNA）表达。使用定量实时 PCR 在细胞和组织标本中验证四个下调和四个上调的 lncRNA 的表达水平。

结果

在这项研究中，与 ANT 肺组织相比，无淋巴结转移的 LAD 中有 949 个 lncRNA 和 681 个 mRNA 表达差异，而有淋巴结转移的 LAD 与无淋巴结转移的 LAD 相比，有 2740 个 lncRNA 和 1714 个 mRNA 表达差异。所选 lncRNA（LINC00113、AC005009.1、ARHGAP22-IT1、AC009411.1、SRGAP3-AS2、EGFEM1P、FAM66E 和 HLA-F-AS1）的表达模式与微阵列数据一致。差异表达的 mRNA 基因富集在关键的基因本体论术语和途径中。

结论

我们的结果揭示了 LAD 中差异表达的 lncRNA，表明 lncRNA 可能是 LAD 诊断和治疗的潜在指标。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f490/6166069/ee6e35a1b471/TCA-9-1312-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f490/6166069/04db0a3c7837/TCA-9-1312-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f490/6166069/7c91f65d9962/TCA-9-1312-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f490/6166069/279a3723fdcb/TCA-9-1312-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f490/6166069/1ce938a715d3/TCA-9-1312-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f490/6166069/98bbfe8ebbd0/TCA-9-1312-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f490/6166069/ee6e35a1b471/TCA-9-1312-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f490/6166069/04db0a3c7837/TCA-9-1312-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f490/6166069/7c91f65d9962/TCA-9-1312-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f490/6166069/279a3723fdcb/TCA-9-1312-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f490/6166069/1ce938a715d3/TCA-9-1312-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f490/6166069/98bbfe8ebbd0/TCA-9-1312-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f490/6166069/ee6e35a1b471/TCA-9-1312-g011.jpg

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