Zhou Ling, Tu Jiangyi, Fang Guangbi, Deng Li, Gao Xiaoqing, Guo Kan, Kong Jiming, Lv Jing, Guan Weikang, Yang Chaoxian
Department of Endocrinology, The Affiliated Hospital, Southwest Medical University, Taiping Street, Luzhou 646000, China.
Department of Anatomy, Southwest Medical University, Zhongshan Road, Luzhou 646000, China.
Stem Cells Int. 2018 Aug 5;2018:5024175. doi: 10.1155/2018/5024175. eCollection 2018.
Nerve tissue engineering is an important strategy for the treatment of brain injuries. Mesenchymal stem cell (MSC) transplantation has been proven to be able to promote repair and functional recovery of brain damage, and poly (lactic-co-glycolic acid) (PLGA) has also been found to have the capability of bearing cells. In the present study, to observe the ability of PLGA scaffold in supporting the adherent growth of MSCs and neurons in vivo and vitro and to assess the effects of PLGA scaffold on proliferation and neural differentiation of MSCs, this study undertakes the following steps. First, MSCs and neurons were cultured and labeled with green fluorescent protein (GFP) or otherwise identified and the PLGA scaffold was synthesized. Next, MSCs and neurons were inoculated on PLGA scaffolds and their adhesion rates were investigated and the proliferation of MSCs was evaluated by using MTT assay. After MSCs were induced by a neural induction medium, the morphological change and neural differentiation of MSCs were detected using scanning electron microscopy (SEM) and immunocytochemistry, respectively. Finally, cell migration and adhesion in the PLGA scaffold in vivo were examined by immunohistochemistry, nuclear staining, and SEM. The experimental results demonstrated that PLGA did not interfere with the proliferation and neural differentiation of MSCs and that MSCs and neuron could grow and migrate in PLGA scaffold. These data suggest that the MSC-PLGA complex may be used as tissue engineering material for brain injuries.
神经组织工程是治疗脑损伤的一项重要策略。间充质干细胞（MSC）移植已被证明能够促进脑损伤的修复和功能恢复，并且聚乳酸-乙醇酸共聚物（PLGA）也已被发现具有承载细胞的能力。在本研究中，为了观察PLGA支架在体内外支持MSC和神经元贴壁生长的能力，并评估PLGA支架对MSC增殖和神经分化的影响，本研究采取了以下步骤。首先，培养MSC和神经元并用绿色荧光蛋白（GFP）进行标记或进行其他鉴定，同时合成PLGA支架。接下来，将MSC和神经元接种到PLGA支架上，研究它们的黏附率，并使用MTT法评估MSC的增殖情况。在用神经诱导培养基诱导MSC后，分别使用扫描电子显微镜（SEM）和免疫细胞化学检测MSC的形态变化和神经分化情况。最后，通过免疫组织化学、核染色和SEM检查PLGA支架在体内的细胞迁移和黏附情况。实验结果表明，PLGA不干扰MSC的增殖和神经分化，并且MSC和神经元能够在PLGA支架中生长和迁移。这些数据表明，MSC-PLGA复合物可作为脑损伤的组织工程材料。
