Sun Sha, Yao Huaxin, Zhang Feifei, Zhu Jin
Department of Polymer Science and Engineering , School of Chemistry and Chemical Engineering , State Key Laboratory of Coordination Chemistry , Nanjing National Laboratory of Microstructures , Nanjing University , Nanjing 210093 , China . Email:
Chem Sci. 2015 Feb 1;6(2):930-934. doi: 10.1039/c4sc02696a. Epub 2014 Oct 15.
Current multiplexed analysis methods suffer from either slow reaction kinetics (planar arrays) or complicated encoding/decoding procedures (suspension arrays). We report herein a multiplexed DNA detection strategy that addresses these issues, based on positional encoding/decoding with self-assembled DNA nanostructures. The strategy enables the acquisition of high-resolution, consistent, and quantitative assay results in a single round of a transmission electron microscopy imaging operation. Applications in polymerase chain reaction-free settings and assays of other structurally distinct targets can be anticipated through the implementation of the strategy with miniaturized femtoliter/attoliter dispensing technology and readily accessible DNA conjugate structures.
当前的多重分析方法要么存在反应动力学缓慢的问题（平面阵列），要么存在复杂的编码/解码程序（悬浮阵列）。我们在此报告一种基于自组装DNA纳米结构的位置编码/解码的多重DNA检测策略，该策略解决了这些问题。该策略能够在一轮透射电子显微镜成像操作中获得高分辨率、一致且定量的检测结果。通过将该策略与微型飞升/阿托升分配技术以及易于获得的DNA共轭结构相结合，可以预期在无聚合酶链反应的环境中的应用以及对其他结构不同的靶标的检测。
