Realpe-Quintero Mauricio, Barba-León Jeannette, Pérez-Montaño Julia A, Pacheco-Gallardo Carlos, González-Aguilar Delia, Dominguez-Arias Rosa M, Cabrera-Diaz Elisa
Universidad de Guadalajara, Departamento de Medicina Veterinaria, Centro Universitario de Ciencias Biológicas y Agropecuarias, Zapopan, Jalisco, Mexico.
Universidad de Guadalajara, Departamento de Salud Pública, Centro Universitario de Ciencias Biológicas y Agropecuarias, Zapopan, Jalisco, Mexico.
PeerJ. 2018 Aug 23;6:e5482. doi: 10.7717/peerj.5482. eCollection 2018.
is one of the major foodborne pathogens worldwide. The antimicrobial resistance (AMR) of this foodborne pathogen has raised a great concern in recent years. Studies on the frequency and characterization of serotypes can help to improve our knowledge on the epidemiology of this pathogen. The purpose of this study was to compare the serotypes, AMR and genetic profiles of isolates recovered from raw beef throughout the beef production chain and from human feces associated with clinical cases of salmonellosis. The serotype, AMR and pulsed-field gel electrophoresis profile of 243 isolates recovered from beef carcasses ( = 78), ground beef ( = 135), and human feces from clinical cases of salmonellosis ( = 30) were compared. Forty-three different serotypes were identified and regardless of the source, the top five corresponded to Typhimurium, Give, Group B (partially serotyped), Infantis and Anatum. Twelve serotypes from beef carcasses were also found in ground beef, showing their presence throughout the beef production chain. Typhimurium, Infantis, Anatum and Montevideo were the only serotypes identified in all sample types. Resistance to tetracyclines was the most frequent (41.2%) followed by resistance to aminoglycosides (37%), folate pathway inhibitors (21%), quinolones (20.2%), phenicols (17.1%), penicillins (15.6%) and cephems (7%). Multidrug resistance was observed in 28.8% of the isolates, and those from human feces showed resistance to a larger number of antimicrobials. Great concern arises from the resistance and reduced susceptibility observed to quinolones and cephalosporins because these drugs are the first line of treatment for invasive infections. Twenty-seven distinct pulse-types were detected among 238 isolates. Clustering analysis for the most frequent serotypes identified groups of isolates with similar AMR profiles. Multidrug resistance spreading throughout the food production chain should be continually monitored and its importance emphasized.
是全球主要的食源性病原体之一。近年来，这种食源性病原体的抗菌药物耐药性（AMR）引起了极大关注。对血清型频率和特征的研究有助于增进我们对该病原体流行病学的了解。本研究的目的是比较从整个牛肉生产链的生牛肉以及与沙门氏菌病临床病例相关的人类粪便中分离出的菌株的血清型、AMR和基因图谱。比较了从牛肉胴体（n = 78）、碎牛肉（n = 135）和沙门氏菌病临床病例的人类粪便（n = 30）中分离出的243株菌株的血清型、AMR和脉冲场凝胶电泳图谱。鉴定出43种不同的血清型，无论来源如何，排名前五的血清型分别是鼠伤寒沙门氏菌、吉韦沙门氏菌、B组（部分血清型）、婴儿沙门氏菌和鸭沙门氏菌。在碎牛肉中也发现了来自牛肉胴体的12种血清型，表明它们在整个牛肉生产链中都存在。鼠伤寒沙门氏菌、婴儿沙门氏菌、鸭沙门氏菌和蒙得维的亚沙门氏菌是在所有样本类型中都鉴定出的仅有的血清型。对四环素的耐药性最为常见（41.2%），其次是对氨基糖苷类（37%）、叶酸途径抑制剂（21%）、喹诺酮类（20.2%）、氯霉素类（17.1%）、青霉素类（15.6%）和头孢菌素类（7%）的耐药性。在28.8%的分离株中观察到多重耐药性，并且来自人类粪便的分离株对更多种类的抗菌药物表现出耐药性。对喹诺酮类和头孢菌素类观察到的耐药性和敏感性降低令人高度担忧，因为这些药物是侵袭性感染的一线治疗药物。在238株分离株中检测到27种不同的脉冲型。对最常见血清型的聚类分析确定了具有相似AMR图谱的分离株组。应持续监测在整个食品生产链中传播的多重耐药性，并强调其重要性。
