Kumada Y, Horinouchi S, Uozumi T, Beppu T
Gene. 1986;42(2):221-4. doi: 10.1016/0378-1119(86)90300-8.
A Streptomyces bikiniensis DNA fragment complementing a deficiency in streptomycin (Sm) production was cloned on the plasmid vector pIJ385. Host strain S. bikiniensis SD 1 used as the recipient in cloning displayed deficiency in biosynthesis of N-methyl-L-glucosamine, one of the moieties of Sm. The cloned fragment on the multicopy plasmid pIJ385 conferred sevenfold increase in Sm production in comparison with the wild-type parental strain. By subcloning, the region complementing the Sm deficiency of SD 1 was narrowed to a 3.0-kb fragment.
一个能弥补比基尼链霉菌链霉素（Sm）产生缺陷的DNA片段被克隆到质粒载体pIJ385上。克隆时用作受体的宿主菌株比基尼链霉菌SD 1在N-甲基-L-葡萄糖胺（Sm的组成部分之一）的生物合成方面存在缺陷。多拷贝质粒pIJ385上克隆的片段使Sm产量与野生型亲本菌株相比提高了七倍。通过亚克隆，弥补SD 1的Sm缺陷的区域被缩小到一个3.0 kb的片段。
