Goosen N, van de Putte P
J Bacteriol. 1986 Aug;167(2):503-7. doi: 10.1128/jb.167.2.503-507.1986.
The Ner protein of bacteriophage Mu negatively regulates transcription initiated at the early promoter and at the major repressor promoter. The construction and isolation of a Ner- mutant of Mu is described. Ner is an essential function for Mu, because the mutant phage only forms plaques when complemented for Ner. Mutations in the repressor protein did not abolish the need for Ner. However, when transcription of the repressor gene c was blocked by deleting the major repressor promoter, Ner was no longer essential for normal Mu development.
噬菌体Mu的Ner蛋白对早期启动子和主要阻遏物启动子起始的转录起负调控作用。本文描述了Mu的Ner突变体的构建与分离。Ner对Mu来说是一项必需功能，因为只有在对Ner进行互补时，突变噬菌体才能形成噬菌斑。阻遏蛋白中的突变并未消除对Ner的需求。然而，当通过删除主要阻遏物启动子来阻断阻遏基因c的转录时，Ner对正常的Mu发育就不再是必需的了。
