Sarabi Asiabar Akram, Asadzadeh Aghdaei Hamid, Sabokbar Azar, Zali Mohammad Reza, Feizabadi Mohammad Mehdi
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.
Basic and Molecular Epidemiology of Gastroenterology Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Med J Islam Repub Iran. 2018 Feb 11;32:11. doi: 10.14196/mjiri.32.11. eCollection 2018.
Crohn's disease and Ulcerative colitis are known as inflammatory bowel disease with high morbidity which are as a result of increasing immune responses to intestinal microbiota in genetically susceptible individuals. The association of adherent invasive with Crohn's disease in human has been discussed for decades. The principal aim of this study was to assess the relationship between adherent invasive in Iranian patients with Crohn's disease. The presence of adherent invasive DNA and viable adherent invasive cells were identified through PCR and conventional culture methods, respectively. All the specimens were subsequently cultured in Hi Chrome Agar medium. Using molecular assay, the invasive plasmid antigen H and invasion-association locus genes were detected from tissue samples confirming the presence of adherent-invasive . The invasive plasmid antigen H was detected in 46.7% of CD and 13.3% of healthy peoples. The invasion-association locus gene was found in 36.7% of patients with Crohn's disease and 10% in individuals without IBD. This study demonstrated an increased frequency of adherent invasive with invasive plasmid antigen H and invasion-association locus genes from patients with CD in comparison to control individuals. Moreover, it was shown that adherent invasive with the invasive plasmid antigen H and invasion-association locus genes can act as a predisposing factor in the development of IBD.
克罗恩病和溃疡性结肠炎被认为是发病率较高的炎症性肠病，它们是由遗传易感个体对肠道微生物群的免疫反应增加所致。人类中侵袭性大肠杆菌与克罗恩病的关联已被讨论了数十年。本研究的主要目的是评估伊朗克罗恩病患者中侵袭性大肠杆菌的关系。侵袭性大肠杆菌DNA的存在和有活力的侵袭性大肠杆菌细胞分别通过聚合酶链反应（PCR）和传统培养方法进行鉴定。所有标本随后在Hi Chrome琼脂培养基中培养。使用分子分析，从组织样本中检测到侵袭性质粒抗原H和侵袭相关位点基因，证实了侵袭性大肠杆菌的存在。在46.7%的克罗恩病患者和13.3%的健康人群中检测到侵袭性质粒抗原H。在36.7%的克罗恩病患者和10%的非炎症性肠病个体中发现了侵袭相关位点基因。与对照个体相比，本研究表明克罗恩病患者中携带侵袭性质粒抗原H和侵袭相关位点基因的侵袭性大肠杆菌频率增加。此外，研究表明，携带侵袭性质粒抗原H和侵袭相关位点基因的侵袭性大肠杆菌可作为炎症性肠病发展的一个易感因素。
